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关于甲醛诱导的5-羟色胺反应产物的显微分光荧光测定研究。

Microspectrofluorometric studies on the formaldehyde-induced reaction product of 5-hydroxytryptamine.

作者信息

Schofield G C, Wreford N G

出版信息

J Microsc. 1979 Jul;116(2):185-98. doi: 10.1111/j.1365-2818.1979.tb00201.x.

DOI:10.1111/j.1365-2818.1979.tb00201.x
PMID:45274
Abstract

A new model incorporating Sephadex G-25 as an inert carrier has been developed for studying the reaction between biogenic amines and formaldehyde vapour. Both the fluorescence yield and the spectral characteristics of the 5-HT formaldehyde reaction product are influenced appreciably by the relative humidity at which paraformaldehyde is equilibrated, the duration of the reaction, the glycine concentration and pH of the suspending solution and the duration of exposure to ultraviolet light. It is concluded that care should be exercised in attempting to identify or quantify 5-HT on the basis of microspectrofluorometric analysis.

摘要

一种以葡聚糖凝胶G - 25作为惰性载体的新模型已被开发出来，用于研究生物胺与甲醛蒸气之间的反应。5 - 羟色胺甲醛反应产物的荧光产率和光谱特征均受到多聚甲醛平衡时的相对湿度、反应持续时间、悬浮液中甘氨酸浓度和pH值以及紫外线照射持续时间的显著影响。得出的结论是，在试图基于显微分光荧光分析来鉴定或定量5 - 羟色胺时应谨慎行事。

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Microspectrofluorometric studies on the formaldehyde-induced reaction product of 5-hydroxytryptamine.关于甲醛诱导的5-羟色胺反应产物的显微分光荧光测定研究。
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引用本文的文献

1
Factors influencing the fluorescence spectra of the formaldehyde-induced reaction product of 5-hydroxytryptamine.影响5-羟色胺甲醛诱导反应产物荧光光谱的因素。
Histochem J. 1980 Sep;12(5):543-52. doi: 10.1007/BF01011928.
2
Standardization of formaldehyde-induced fluorescence and its measurement to quantify serotonin emission in pulmonary neuroendocrine cells.甲醛诱导荧光的标准化及其测量以量化肺神经内分泌细胞中的5-羟色胺释放
Histochemistry. 1982;75(2):251-8. doi: 10.1007/BF00496015.
3
Microspectrofluorometric characterization of the fluorescent derivatives of biogenic amines produced by aqueous aldehyde (Faglu) fixation.通过水性醛（荧光胺）固定产生的生物胺荧光衍生物的显微分光荧光特性分析。
Histochem J. 1982 May;14(3):491-505. doi: 10.1007/BF01011859.
4
Computer-operated microspectrofluorimetry to identify formaldehyde-induced fluorophores of biogenic monoamines and precursor substances in models and tissue sections.计算机操作的显微分光荧光测定法用于鉴定模型和组织切片中甲醛诱导的生物源性单胺及其前体物质的荧光团。
Histochemistry. 1987;86(3):255-67. doi: 10.1007/BF00490256.
5
Differentiation of the formaldehyde-induced fluorescent products of noradrenaline and dopamine by microspectrofluorometry.
Histochem J. 1979 Jul;11(4):473-83. doi: 10.1007/BF01002774.

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