Fuchs S, Aharonov A, Sela M, von der Haar F, Cramer F
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2800-2. doi: 10.1073/pnas.71.7.2800.
Antibodies with specificity to a single species of tRNA were elicited in a goat by immunization with a glutaraldehyde conjugate of yeast phenylalanine transfer RNA with bovine gamma globulin. The specificity of the antibodies was studied by a radioimmunoassay measuring the direct binding of [(3)H]tRNA(Phe) or the inhibition of the binding. The antibodies formed are predominantly directed towards the characteristic highly modified nucleoside Y, which is located right next to the anticodon. The antibodies bind specifically to tRNA(Phe), to oligonucleotides derived by enzymatic digestion from the anticodon loop of tRNA(Phe), and to the Y nucleoside itself. tRNA species which do not contain Y in their sequences, or tRNA(Phe) from which the Y base has been excised, do not bind to the antibodies. Yeast tRNA(Phe) can be separated from other tRNA species with an immunoadsorbent of antibodies to tRNA(Phe).
用酵母苯丙氨酸转移RNA与牛γ球蛋白的戊二醛偶联物免疫山羊，可诱导产生对单一tRNA物种具有特异性的抗体。通过放射免疫测定法测量[(3)H]tRNA(Phe)的直接结合或其结合抑制来研究抗体的特异性。所形成的抗体主要针对紧邻反密码子的特征性高度修饰核苷Y。这些抗体特异性地结合tRNA(Phe)、从tRNA(Phe)反密码子环经酶切得到的寡核苷酸以及Y核苷本身。序列中不含Y的tRNA物种，或已切除Y碱基的tRNA(Phe)，均不与抗体结合。酵母tRNA(Phe)可用针对tRNA(Phe)的抗体免疫吸附剂与其他tRNA物种分离。
