Valenzuela P, Venegas A, Weinberg F, Bishop R, Rutter W J
Proc Natl Acad Sci U S A. 1978 Jan;75(1):190-4. doi: 10.1073/pnas.75.1.190.
Sixteen bacterial clones containing sequences complementary to yeast PhetRNA were isolated from a collection of hybrid plasmids containing BamHI restriction endonuclease-generated yeast DNA fragments inserted in the plasmid vector pBR315. Ten of these clones contained hybrid plasmids with distinct BamHI fragments. The sequence of the Phe-tRNA structural genes and adjacent regions of three of these clones is reported here. In the region flanking the tRNA gene, the sequence of two of the cloned DNAs is similar; the sequence of the third varies considerably. All three of the tRNA genes are bordered by A,T-rich regions. In particular, near the region coding for the 3' end of the tRNA there is a long sequence of As in the coding strand. This is reminiscent of the region of termination of transcription of the yeast 5S rRNA gene. The sequences coding for the Phe-tRNA contain an additional segment of 18 or 19 base pairs (depending upon the clone) not predicted by the yeast Phe-tRNA sequence. These intervening segments are nearly identical in the three clones and are located within the structural gene, two base pairs from the nucleotides coding for the tRNA anticodon.
从一组含有插入质粒载体pBR315的经BamHI限制性内切酶切割产生的酵母DNA片段的杂交质粒中，分离出16个含有与酵母苯丙氨酸转运RNA（Phe-tRNA）序列互补的细菌克隆。其中10个克隆含有带有不同BamHI片段的杂交质粒。本文报道了其中3个克隆的苯丙氨酸转运RNA结构基因及相邻区域的序列。在转运RNA基因侧翼区域，两个克隆DNA的序列相似；第三个克隆的序列差异较大。所有3个转运RNA基因都由富含A、T的区域界定。特别地，在编码转运RNA 3'端的区域附近，编码链上有一段长的A序列。这使人联想到酵母5S核糖体RNA基因的转录终止区域。编码苯丙氨酸转运RNA的序列包含一段18或19个碱基对的额外片段（取决于克隆），这是酵母苯丙氨酸转运RNA序列未预测到的。这些居间序列在3个克隆中几乎相同，且位于结构基因内，距离编码转运RNA反密码子的核苷酸两个碱基对处。
