在与叶酸一起孵育的大鼠肝切片中形成10-甲酰四氢叶酸，一种二氢叶酸还原酶的有效抑制剂。
Formation of 10-formylfolic acid, a potent inhibitor of dihydrofolate reductase, in rat liver slices incubated with folic acid.
作者信息
D'Urso-Scott M, Uhoch J, Bertino J R
出版信息
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2736-9. doi: 10.1073/pnas.71.7.2736.
Abstract
During investigation of folate polyglutamate biosynthesis in rat liver slices utilizing [2-(14)C]folic acid, a folate compound that behaved like a polyglutamate form in the Sephadex G-15 gel filtration system was found to accumulate. Subsequent chromatographic, spectral, chemical, and enzymic studies have indicated that the compound formed in liver slices incubated with [(14)C]folic acid with and without methotrexate was 10-formyl folate. This folate is of interest in that it is the most potent natural inhibitor of dihydrofolate reductase known and may be capable of serving a regulatory function within the cell.
摘要
在利用[2-(14)C]叶酸对大鼠肝切片中叶酸多聚谷氨酸生物合成进行研究的过程中，发现有一种叶酸化合物在葡聚糖G-15凝胶过滤系统中表现得像多聚谷氨酸形式并发生了积累。随后的色谱、光谱、化学和酶学研究表明，在有和没有甲氨蝶呤的情况下与[(14)C]叶酸一起孵育的肝切片中形成的化合物是10-甲酰基叶酸。这种叶酸之所以令人感兴趣，是因为它是已知的最有效的二氢叶酸还原酶天然抑制剂，并且可能能够在细胞内发挥调节功能。
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