放射性标记叶酸示踪剂给药48小时后在大鼠肝脏中发现的叶酸共轭物的鉴定。
The identification of the folate conjugates found in rat liver 48 h after the administration of radioactively labelled folate tracers.
作者信息
Connor M J, Blair J A
出版信息
Biochem J. 1980 Jan 15;186(1):235-42. doi: 10.1042/bj1860235.
About 70% of the radioactivity retained in the livers of rats dosed 48 h earlier with radioactively labelled folate was incorporated into two folate conjugates. The major derivative was purified and isolated by Sephadex G-15, DEAE-cellulose and DEAE-Sephadex ion-exchange column chromatography and paper chromatography. It was identified as 10-formylpteroylpentaglutamate by a combination of spectral, microbiological, chemical and chromatographic techniques. The minor conjugate, though less well characterized, exhibited similar properties and was assigned the structure 10-formylpteroyltetraglutamate. 10-Formylpteroylpentaglutamate (2.0nmol/g) and 10-formylpteroyltetraglutamate (0.25nmol/g) comprised about 20% of the total endogenous hepatic folate as determined by microbiological assay (Lactobacillus casei after conjugase treatment.
48小时前用放射性标记叶酸给药的大鼠肝脏中保留的约70%放射性被整合到两种叶酸共轭物中。主要衍生物通过葡聚糖凝胶G-15、二乙氨基乙基纤维素和二乙氨基乙基葡聚糖离子交换柱色谱法以及纸色谱法进行纯化和分离。通过光谱、微生物学、化学和色谱技术相结合的方法，将其鉴定为10-甲酰蝶酰五谷氨酸。次要共轭物虽然表征程度较低，但表现出相似的性质，并被赋予10-甲酰蝶酰四谷氨酸的结构。通过微生物测定法（结合酶处理后的干酪乳杆菌）测定，10-甲酰蝶酰五谷氨酸（2.0nmol/g）和10-甲酰蝶酰四谷氨酸（0.25nmol/g）约占肝脏内源性叶酸总量的20%。
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