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多肽二级结构的碳-13磁共振评估及其与质子磁共振研究的相关性。

Carbon-13 magnetic resonance evaluation of polypeptide secondary structure and correlation with proton magnetic resonance studies.

作者信息

Urry D W, Mitchell L W, Onishi T

出版信息

Proc Natl Acad Sci U S A. 1974 Aug;71(8):3265-9. doi: 10.1073/pnas.71.8.3265.

DOI:10.1073/pnas.71.8.3265
PMID:4528456
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388665/
Abstract

With the use of appropriately chosen solvent pairs it is demonstrated that solvent dependence of peptide carbonyl carbon resonances can be correlated with polypeptide secondary structure. Solvent titrations show the peptide carbonyl which is intramolecularly hydrogen bonded to exhibit less chemical shift on going from a dimethylsulfoxide solution to a solution containing a solvent which is a good proton (or deuteron) donor. Effective solvent systems are dimethylsulfoxide paired with water, trifluoroethanol, or methanol. This approach is demonstrated with the pentapeptide of elastin.

摘要

通过使用适当选择的溶剂对，已证明肽羰基碳共振的溶剂依赖性可与多肽二级结构相关联。溶剂滴定表明，与分子内形成氢键的肽羰基从二甲基亚砜溶液转变为含有良好质子（或氘核）供体的溶剂的溶液时，化学位移变化较小。有效的溶剂体系是二甲基亚砜与水、三氟乙醇或甲醇配对。用弹性蛋白的五肽证明了这种方法。

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本文引用的文献

1
Temperature dependence of amide proton chemical shifts: the secondary structures of gramicidin S and valinomycin.酰胺质子化学位移的温度依赖性：短杆菌肽S和缬氨霉素的二级结构
Biochem Biophys Res Commun. 1969 Jul 23;36(2):194-202. doi: 10.1016/0006-291x(69)90314-3.
2
The molecular structure and some transport properties of valinomycin.缬氨霉素的分子结构及一些转运特性。
Biochem Biophys Res Commun. 1969 May 22;35(4):512-8. doi: 10.1016/0006-291x(69)90376-3.
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Conformations of cyclic peptides. IV. Nuclear magnetic resonance studies of cyclo-pentaglycyl-L-leucyl and cyclo-diglycyl-L-histidyldiglycyl-L-tyrosyl.环肽的构象。IV. 环戊基甘氨酰-L-亮氨酰和环二甘氨酰-L-组氨酰二甘氨酰-L-酪氨酰的核磁共振研究。
Biochemistry. 1969 Oct;8(10):4087-95. doi: 10.1021/bi00838a028.
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Prolactin synthesis in primates.灵长类动物中催乳素的合成。
Nat New Biol. 1971 Jul 7;232(27):18-9.
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Proposed conformation of oxytocin in solution.溶液中催产素的推测构象。
Proc Natl Acad Sci U S A. 1971 May;68(5):956-8. doi: 10.1073/pnas.68.5.956.
6
Secondary structure of the cyclic moiety of the peptide hormone oxytocin and its deamino analog.肽激素催产素及其脱氨基类似物环状部分的二级结构。
Proc Natl Acad Sci U S A. 1970 May;66(1):111-6. doi: 10.1073/pnas.66.1.111.
7
A conformational analysis of gramicidin S-A by nuclear magnetic resonance.通过核磁共振对短杆菌肽S-A进行构象分析。
Proc Natl Acad Sci U S A. 1968 Oct;61(2):734-41. doi: 10.1073/pnas.61.2.734.
8
Proton magnetic resonance studies in trifluoroethanol. Solvent mixtures as a means of delineating peptide protons.三氟乙醇中的质子磁共振研究。以溶剂混合物作为描绘肽质子的一种手段。
J Am Chem Soc. 1972 Feb 23;94(4):1399-400. doi: 10.1021/ja00759a083.
9
Studies on the conformation and interactions of elastin. Proton magnetic resonance of the repeating pentapeptide.弹性蛋白的构象与相互作用研究。重复五肽的质子磁共振。
Biochemistry. 1974 Jan 29;13(3):609-16. doi: 10.1021/bi00700a032.
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Carbon-13 nuclear magnetic resonance spectroscopy of oxytocin, related oligopeptides, and selected analogs.催产素、相关寡肽及选定类似物的碳-13核磁共振波谱分析
Biochemistry. 1973 Apr 10;12(8):1643-9. doi: 10.1021/bi00732a028.