Urry D W, Mitchell L W, Onishi T
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3265-9. doi: 10.1073/pnas.71.8.3265.
With the use of appropriately chosen solvent pairs it is demonstrated that solvent dependence of peptide carbonyl carbon resonances can be correlated with polypeptide secondary structure. Solvent titrations show the peptide carbonyl which is intramolecularly hydrogen bonded to exhibit less chemical shift on going from a dimethylsulfoxide solution to a solution containing a solvent which is a good proton (or deuteron) donor. Effective solvent systems are dimethylsulfoxide paired with water, trifluoroethanol, or methanol. This approach is demonstrated with the pentapeptide of elastin.
通过使用适当选择的溶剂对,已证明肽羰基碳共振的溶剂依赖性可与多肽二级结构相关联。溶剂滴定表明,与分子内形成氢键的肽羰基从二甲基亚砜溶液转变为含有良好质子(或氘核)供体的溶剂的溶液时,化学位移变化较小。有效的溶剂体系是二甲基亚砜与水、三氟乙醇或甲醇配对。用弹性蛋白的五肽证明了这种方法。