Deppert W, Werchau H, Walter G
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3068-72. doi: 10.1073/pnas.71.8.3068.
Intact BHK (baby hamster kidney) cells catalyze the hydrolysis of UDP-galactose to free galactose. The generation of galactose from UDP-galactose and its intracellular utilization impede the detection of possible galactosyl transferases on the cell surface of intact cells. Several independent procedures have been used to distinguish between intracellular and cell surface glycosyl transferases. With these procedures, no evidence was obtained for the presence of detectable amounts of galactosyl transferase activity on the surface of BHK cells. The data suggest that galactosyl transferases do not play a general role in the phenomena of cell adhesion and contact inhibition.
完整的幼仓鼠肾(BHK)细胞催化UDP-半乳糖水解为游离半乳糖。UDP-半乳糖生成半乳糖及其在细胞内的利用,阻碍了对完整细胞表面可能存在的半乳糖基转移酶的检测。已经使用了几种独立的方法来区分细胞内和细胞表面的糖基转移酶。通过这些方法,未获得证据表明BHK细胞表面存在可检测量的半乳糖基转移酶活性。数据表明,半乳糖基转移酶在细胞黏附和接触抑制现象中不发挥普遍作用。