Wreschner D H, James T C, Silverman R H, Kerr I M
Nucleic Acids Res. 1981 Apr 10;9(7):1571-81. doi: 10.1093/nar/9.7.1571.
Ribosomal RNA (rRNA) in intact ribosomes is cleaved into discrete products on incubation of reticulocyte lysates or L-cell extracts with ppp(A2'p)3A. Cleavage of rRNA may, therefore, provide a useful assay for 2-5A (ppp)A2'p)nA; n = 2 to 4) or for the presence of a 2-5A-dependent nuclease. The results with reticulocyte lysates differed from those obtained in the L-cell-free system in that (a) a different RNA cleavage pattern was produced (with added L-cell ribosomes) and (b) cleavage was fully activated by the analogue ppp(A2'p)3A3'pCp. As might be expected from the relatively high levels of 2-5A present in interferon-treated, encephalomyocarditis virus (EMC)-infected L-cells, rRNA extracted from these cells was also cleaved. The cleavage pattern observed overlapped with that obtained on incubation of an L-cell-free system with 2-5A. Thus, not only is 2-5A present, but the 2-5A-dependent nuclease also appears to be active, in interferon-treated, EMC-infected L-cells.
在网织红细胞裂解物或L细胞提取物与ppp(A2'p)3A共同孵育时，完整核糖体中的核糖体RNA（rRNA）会被切割成离散的产物。因此，rRNA的切割可为2-5A（ppp(A2'p)nA；n = 2至4）或2-5A依赖性核酸酶的存在提供一种有用的检测方法。网织红细胞裂解物的结果与无L细胞系统中获得的结果不同，在于（a）产生了不同的RNA切割模式（添加L细胞核糖体时），以及（b）类似物ppp(A2'p)3A3'pCp可完全激活切割。正如从经干扰素处理的、感染脑心肌炎病毒（EMC）的L细胞中相对较高水平的2-5A所预期的那样，从这些细胞中提取的rRNA也被切割。观察到的切割模式与用2-5A孵育无L细胞系统时获得的模式重叠。因此，在经干扰素处理的、感染EMC的L细胞中，不仅存在2-5A，而且2-5A依赖性核酸酶似乎也具有活性。
Zotero 插件
