Van Ramshorst J D, Sundaresan T K, Outschoorn A S
Bull World Health Organ. 1972;46(2):263-76.
Collaborative studies showed that relative potency assays for a particular type of diphtheria toxoid (adsorbed) and for tetanus toxoid (plain and adsorbed) gave very similar results, whether the assays were carried out by toxin challenge or by antitoxin titration after immunization of experimental animals with graded doses of toxoid. The same numerical results were obtained with a scoring system as with a system based on survivals only. Although skin tests were used on a very limited scale in these studies, it seems likely that they could replace lethal tests for the diphtheria challenge assays.For both tetanus and diphtheria, the adsorbed toxoid gave a higher relative potency when combined with other antigens than as a single toxoid. Both mice and guinea-pigs were used for the lethal challenge test of adsorbed tetanus toxoid. For the single tetanus toxoid the results were the same, but for the combined toxoid (DPT vaccine) the mouse assay results were about twice those of guinea-pig assays.
合作研究表明，对于特定类型的吸附白喉类毒素和破伤风类毒素（普通和吸附型），无论通过毒素攻击还是在用分级剂量类毒素免疫实验动物后进行抗毒素滴定来进行相对效价测定，结果都非常相似。使用评分系统和仅基于存活率的系统得到了相同的数值结果。尽管在这些研究中皮肤试验的使用规模非常有限，但似乎它们可以替代白喉攻击试验中的致死试验。对于破伤风和白喉，吸附类毒素与其他抗原联合使用时的相对效价比单独作为类毒素时更高。小鼠和豚鼠都用于吸附破伤风类毒素的致死攻击试验。对于单一破伤风类毒素，结果相同，但对于联合类毒素（百白破疫苗），小鼠试验结果约为豚鼠试验结果的两倍。
