Schwartz J E, Durham B C
Ann Clin Lab Sci. 1979 Mar-Apr;9(2):139-43.
The Biuret, Ultraviolet and Lowry techniques for the determination of total protein content were compared in 31 samples of patient serum and in 30 rat heart tissue homogenates. Statistically significant differences were found to exist between values obtained with each method. Human serum and heart homogenate protein were both lowest when assayed by the Lowry procedure, while serum and homogenate values were highest with the Ultraviolet and Biuret method, respectively. Possible sources of these discrepancies are discussed. Although the Biuret method may not be as sensitive as the other two methods, it proved to be the most reproducible technique for determining total protein concentration in the particular types of samples used in this study.
对31份患者血清样本和30份大鼠心脏组织匀浆，比较了用于测定总蛋白含量的双缩脲法、紫外法和洛瑞法。发现各方法所得数值之间存在统计学显著差异。用洛瑞法检测时，人血清和心脏匀浆蛋白含量均最低，而血清和匀浆数值分别在紫外法和双缩脲法检测时最高。讨论了这些差异可能的来源。虽然双缩脲法可能不如其他两种方法灵敏，但在本研究使用的特定类型样本中，它被证明是测定总蛋白浓度最可重复的技术。
