Hartmann J, Reineke W, Knackmuss H J
Appl Environ Microbiol. 1979 Mar;37(3):421-8. doi: 10.1128/aem.37.3.421-428.1979.
Pseudomonas sp. WR912 was isolated by continuous enrichment in three steps with 3-chloro-, 4-chloro-, and finally 3,5-dichlorobenzoate as sole source of carbon and energy. The doubling times of the pure culture with these growth substrates were 2.6, 3.3, and 5.2 h, respectively. Stoichiometric amounts of chloride were eliminated during growth. Oxygen uptake rates with chlorinated benzoates revealed low stereospecificity of the initial benzoate 1,2-dioxygenation. Dihydrodi-hydroxybenzoate dehydrogenase, catechol 1,2-dixoygenase, and muconate cycloisomerase activities were found in cell-free extracts. The ortho cleavage activity for catechols appeared to involve induction of isoenzymes with different stereospecificity towards chlorocatechols. A catabolic pathway for chlorocatechols was proposed on the basis of similarity to chlorophenoxyacetate catabolism, and cometabolism of 3,5-dimethylbenzoate by chlorobenzoate-induced cells yielded 2,5-dihydro-2,4-dimethyl-5-oxo-furan-2-acetic acid.
假单胞菌属WR912是通过连续三步富集培养分离得到的，这三步分别以3-氯苯甲酸、4-氯苯甲酸，最后以3,5-二氯苯甲酸作为唯一碳源和能源。该纯培养物在这些生长底物上的倍增时间分别为2.6小时、3.3小时和5.2小时。生长过程中化学计量的氯离子被去除。以氯代苯甲酸为底物时的氧气摄取率表明，初始苯甲酸1,2-双加氧酶的立体特异性较低。在无细胞提取物中发现了二氢二羟基苯甲酸脱氢酶、儿茶酚1,2-双加氧酶和粘康酸环异构酶的活性。儿茶酚的邻位裂解活性似乎涉及对氯代儿茶酚具有不同立体特异性的同工酶的诱导。基于与氯苯氧基乙酸分解代谢的相似性，提出了氯代儿茶酚的分解代谢途径，并且氯苯甲酸诱导的细胞对3,5-二甲基苯甲酸的共代谢产生了2,5-二氢-2,4-二甲基-5-氧代-呋喃-2-乙酸。
