Smith S R, Burton D W, Izenstark J L
Arch Intern Med. 1979 Jul;139(7):757-60.
A modified thyroid-stimulating hormone (TSH) radioimmunoassay with particular sensitivity at the bottom end of the normal range was developed using dog serum in standard tubes and reduced quantities of TSH labeled with radioactive iodine (125I) and antiserum. The standard deviation of B0 points was 1%, implying an assay sensitivity of less than 0.1 microU/mL. This procedure was used to measure serum TSH levels in 32 normal subjects, 18 patients with hyperthyroidism, and seven patients with elevation of serum triiodothyronine (T3) levels. The range of values obtained from normal subjects was less than 0.1 to 4.1 microU/mL, with 97% of the subjects' values greater than 0.2 microU/mL. All of the hyperthyroid patients had values less than or equal to 0.2 microU/mL. Hence, the modified TSH assay was thought to be of use in distinguishing hyperthyroid from normal subjects. The TSH levels in subjects with elevated T3 levels ranged from less than 0.1 to 1.8 microU/mL. These results were thought to indicate heterogeneity within this group and suggest that only certain of these individuals merit the diagnosis of "T3 toxicosis."
采用狗血清、减少放射性碘（¹²⁵I）标记促甲状腺激素（TSH）和抗血清的用量，开发了一种在正常范围下限具有特殊灵敏度的改良TSH放射免疫测定法。B0点的标准差为1%，这意味着该测定法的灵敏度小于0.1微单位/毫升。此方法用于测量32名正常受试者、18名甲状腺功能亢进患者和7名血清三碘甲状腺原氨酸（T3）水平升高患者的血清TSH水平。正常受试者的数值范围为小于0.1至4.1微单位/毫升，97%的受试者数值大于0.2微单位/毫升。所有甲状腺功能亢进患者的数值均小于或等于0.2微单位/毫升。因此，改良的TSH测定法被认为可用于区分甲状腺功能亢进患者与正常受试者。T3水平升高的受试者的TSH水平范围为小于0.1至1.8微单位/毫升。这些结果被认为表明该组存在异质性，并提示只有其中某些个体值得诊断为“T3中毒症”。
