Witkin S S, Shahani S K, Gupta S, Good R A, Day N K
Clin Exp Immunol. 1980 Sep;41(3):441-52.
The presence of Fc receptors, but not C3 receptors, on human and bovine spermatozoa was shown by sperm-induced rosette formation of antibody-treated ox erythrocytes and by immunofluorescence of aggregated human gammaglobulin (AHG) treated spermatozoa. Human and bovine spermatozoa bound AHG as well as circulating immune complexes (CIC) from human sera; monomeric IgG bound to these cells to a much lesser extent. Binding of AHG to the sperm surface also occurred when the AHG was suspended in either buffer or in serum that had been heated at 56 degrees C for 30 min. A bovine spermatozoa radioimmunoassay for the detection of immune complexes in human sera was developed and was shown to be comparable to the Raji cell radioimmunoassay (r = 0 . 81, P < 0 . 01) in the quantitation of soluble immune complexes from a variety of human sera. An enzyme-linked immunoassay was then developed, using bovine spermatozoa fixed to wells of microtitre plates, to simplify the immune complex detection assay. The technique provides an analysis that is rapid, does not require radioactive chemicals, expensive equipment, or cultured cells and yields values for immune complex determination both comparable and complementary to the Raji cell radioimmunoassay (r = 0 . 70, P < 0 . 01). The sperm assays detected soluble immune complexes in sera of vasectomized males, cancer patients and patients with autoimmune or dermatological diseases.
通过抗体处理的牛红细胞的精子诱导花环形成以及经聚集人丙种球蛋白（AHG）处理的精子的免疫荧光，证明了人和牛精子上存在Fc受体而非C3受体。人和牛精子能结合AHG以及人血清中的循环免疫复合物（CIC）；单体IgG与这些细胞的结合程度要小得多。当AHG悬浮于缓冲液或经56℃加热30分钟的血清中时，AHG也会与精子表面结合。开发了一种用于检测人血清中免疫复合物的牛精子放射免疫测定法，结果表明，在定量各种人血清中的可溶性免疫复合物时，该方法与Raji细胞放射免疫测定法相当（r = 0.81，P < 0.01）。随后开发了一种酶联免疫测定法，使用固定在微量滴定板孔中的牛精子，以简化免疫复合物检测测定法。该技术提供了一种快速的分析方法，不需要放射性化学物质、昂贵的设备或培养细胞，并且所产生的免疫复合物测定值与Raji细胞放射免疫测定法相当且互补（r = 0.70，P < 0.01）。精子测定法检测到了输精管切除男性、癌症患者以及自身免疫性或皮肤病患者血清中的可溶性免疫复合物。
