Zimmerman T S, Edgington T S
J Exp Med. 1973 Oct 1;138(4):1015-20. doi: 10.1084/jem.138.4.1015.
Factor VIII coagulant activity (VIII(C)) and the von Willebrand's disease antigen (Factor VIII-like antigen, vW-Ag) are biologically linked, and it has been suggested that they reside on the same molecule. However, insolubilized human isoantibody to VIII(C) and rabbit antiserum containing antibodies to VIII(C) and vW-Ag differentially bind and remove these entities from plasma, thus physically segregating one from the other. These findings indicate that Factor VIII coagulant activity resides on a molecule distinct from that expressing the von Willebrand's antigen.
凝血因子VIII促凝活性(VIII(C))与血管性血友病因子抗原(VIII样抗原,vW-Ag)存在生物学联系,有人提出它们存在于同一分子上。然而,针对VIII(C)的不溶性人同种抗体以及含有针对VIII(C)和vW-Ag抗体的兔抗血清对这些物质的结合和从血浆中清除情况不同,从而在物理上使它们彼此分离。这些发现表明,凝血因子VIII促凝活性存在于一个与表达血管性血友病因子抗原的分子不同的分子上。