Green J R, Northcote D H
Biochem J. 1979 Mar 15;178(3):661-71. doi: 10.1042/bj1780661.
Two types of experiments were carried out; either maize roots were incubated in L-[1-3H]fucose or membranes were prepared from root tips and these were incubated with GDP-L-[U-14C]fucose or UDP-D-[U-4C]glucose. The radioactively labelled lipids that were synthesized in vivo and in vitro were extracted and separated into polar and neutral components. The polar lipids had the characteristics of polyprenyl phosphate and diphosphate fucose or glucose derivatives, and the neutral lipids of sterol glycosides (fucose or glucose). A partial separation of the glycolipid synthetase reactions was achieved. Membranes were fractionated into material that sedimented at 20,000g and 100,000g. Most of the polar glycolipid synthetase activity (for the incorporation of both fucose and glucose) was located in the 100,000 g pellet, and this activity was probably located in the endoplasmic reticulum. The neutral lipid, which contained fucose, was synthesized mainly by membranes of the 20,000g pellet, and the activity was probably associated with the dictyosomes, whereas the neutral glucolipids were synthesized by all the membrane fractions. It is suggested that the polar (polyprenyl) lipids labelled with fucose could act as possible intermediates during the synthesis of the glycoproteins and slime in the root tip.
要么将玉米根在L-[1-3H]岩藻糖中孵育，要么从根尖制备膜，并将这些膜与GDP-L-[U-14C]岩藻糖或UDP-D-[U-4C]葡萄糖一起孵育。将体内和体外合成的放射性标记脂质提取出来，并分离成极性和中性成分。极性脂质具有聚异戊二烯磷酸和二磷酸岩藻糖或葡萄糖衍生物的特征，中性脂质为甾醇糖苷（岩藻糖或葡萄糖）。实现了糖脂合成酶反应的部分分离。将膜分级分离成在20,000g和100,000g沉降的物质。大多数极性糖脂合成酶活性（用于掺入岩藻糖和葡萄糖）位于100,000g沉淀中，并且这种活性可能位于内质网中。含有岩藻糖的中性脂质主要由20,000g沉淀的膜合成，并且该活性可能与高尔基体相关，而中性糖脂由所有膜分级分离物合成。有人提出，用岩藻糖标记的极性（聚异戊二烯）脂质可能在根尖糖蛋白和黏液的合成过程中作为可能的中间体。
