玉米（Zea mays）根冠细胞膜在黏液多糖产生过程中合成的糖蛋白的结构与功能。
The structure and function of glycoproteins synthesized during slime-polysaccharide production by membranes of the root-cap cells of maize (Zea mays).
作者信息
Green J R, Northcote D H
出版信息
Biochem J. 1978 Mar 15;170(3):599-608. doi: 10.1042/bj1700599.
Abstract
The synthesis of the maize root slime polysaccharides was investigated by using [1-3H]-fucose as a marker for slime production. Three fractions were separated by centrifugation in a CsCl density gradient. Two of these were glycoproteins and occurred within the membranes of the cells of the root tip; the third was the slime polysaccharides. Radioactive pulse-chase experiments showed that the glycoproteins were precursors of the slime polysaccharides, and the carbohydrate portion of the glycoproteins had a similar composition to that of the free slime. The linkage between the protein and the carbohydrate of one of the glycoproteins was shown to be a xylose-threonine bond. It is postulated that the slime polysaccharides are synthesized and transported on proteins within the membrane system of the root tip.
摘要
以[1-³H]-岩藻糖作为黏液产生的标记物，对玉米根黏液多糖的合成进行了研究。通过在氯化铯密度梯度中离心分离出三个组分。其中两个是糖蛋白，存在于根尖细胞的膜内；第三个是黏液多糖。放射性脉冲追踪实验表明，糖蛋白是黏液多糖的前体，并且糖蛋白的碳水化合物部分与游离黏液的组成相似。其中一种糖蛋白的蛋白质与碳水化合物之间的连接被证明是木糖-苏氨酸键。据推测，黏液多糖是在根尖膜系统内的蛋白质上合成并运输的。
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本文引用的文献
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