Conde R D
Biochem J. 1979 Mar 15;178(3):725-31. doi: 10.1042/bj1780725.
The effect of hypophysectomy on the protein metabolism of the liver in vivo was studied. Fractional rates of protein synthesis and degradation were determined in the livers of normal and hypophysectomized rats. Synthesis was measured after the injection of massive amounts of radioactive leucine. Degradation was estimated either as the balance between synthesis and accumulation of stable liver proteins or from the disappearance of radioactivity from the proteins previously labelled by the injection of NaH14CO3. The results indicate that: (1) hypophysectomy diminishes the capacity of the liver to synthesize proteins in vivo, mainly of those that are exported as plasma proteins; (2) livers of both normal and hypophysectomized rats show identical protein-degradation rates, whereas plasma proteins are degraded slowly after hypophysectomy.
研究了垂体切除对体内肝脏蛋白质代谢的影响。测定了正常大鼠和垂体切除大鼠肝脏中蛋白质合成和降解的分数率。在注射大量放射性亮氨酸后测量合成情况。降解情况通过稳定肝脏蛋白质合成与积累之间的平衡来估计，或者根据先前注射NaH14CO3标记的蛋白质中放射性的消失来估计。结果表明：（1）垂体切除会降低肝脏在体内合成蛋白质的能力，主要是那些作为血浆蛋白输出的蛋白质；（2）正常大鼠和垂体切除大鼠的肝脏显示出相同的蛋白质降解率，而垂体切除后血浆蛋白降解缓慢。
