Bisseling T, van den Bos R C, Weststrate M W, Hakkaart M J, van Kammen A
Biochim Biophys Acta. 1979 May 24;562(3):515-26. doi: 10.1016/0005-2787(79)90114-x.
Some aspects of root nodule development of Pisum sativum inoculated with Rhizobium leguminosarum were examined. 1. Nitrogenase activity (measured as acetylene reduction) appears to be preceded by leghemoglobin synthesis (measured immunologically). 2. Syntheses of component I and component II of nitrogenase are not strictly coordinated. Synthesis of component I starts before component II. 3. Plant and bacteroid protein synthesis (measured by [35S]sulfate labeling) in root nodules declines rapidly during nodule development. Corresponding with this decline is a decrease in quantity and quality of rRNA.
对接种了豌豆根瘤菌的豌豆根瘤发育的某些方面进行了研究。1. 固氮酶活性（以乙炔还原法测定）似乎先于豆血红蛋白合成（以免疫法测定）。2. 固氮酶组分I和组分II的合成并非严格协调。组分I的合成先于组分II开始。3. 根瘤中植物和类菌体蛋白质合成（以[35S]硫酸盐标记法测定）在根瘤发育过程中迅速下降。与此下降相对应的是rRNA数量和质量的降低。
