Dorosinskii L M, Afanas'eva L M, Kutin A A
Mikrobiologiia. 1979 May-Jun;48(3):495-501.
The activity of nitrogenase in nodules of pea plants and the rate of diurnal nitrogen fixation were studied at different phases of the plant growth using the acetylene and isotope techniques. At the same time, the morphology of nodule bacteria was studied by electron and phase-contrast microscopy. About 90% of the bacteria in nodules were found in the form of bacteroids from the early phase of the plant growth (budding) to ripening when gemmated arthrospores could be observed on some bacteroids. The highest activity of nitrogenase and nitrogen fixation was detected at the reproductive stage of growth. Therefore, in order to obtain more active strains of pea nodule bacteria, they should be isolated from nodules at this stage of plant growth.
利用乙炔和同位素技术，研究了豌豆植株根瘤中固氮酶的活性以及不同生长阶段的昼夜固氮速率。同时，通过电子显微镜和相差显微镜研究了根瘤菌的形态。从植株生长早期（出芽期）到成熟期，根瘤中约90%的细菌以类菌体形式存在，此时在一些类菌体上可观察到芽孢。在生殖生长阶段检测到固氮酶活性和固氮作用最高。因此，为了获得更具活性的豌豆根瘤菌菌株，应在植株生长的这一阶段从根瘤中分离它们。
