Mannervik B, Jakobson I, Warholm M
Biochim Biophys Acta. 1979 Mar 16;567(1):43-8. doi: 10.1016/0005-2744(79)90170-0.
The experimental variance of enzymic steady-state kinetic experiments depends on velocity as approximated by a power function (Var(v) = K1 . valpha (Askelöf, P., Korsfeldt, M. and Mannervik, B. (1976) Eur. J. Biochem. 69, 61--67). The values of the constants (K1, alpha) can be estimated by making replicate measurements of velocity, and the inverse of the function can then be used as a weighting factor. In order to avoid measurement of a large number of replicates to establish the error structure of a kinetic data set, a different approach was tested. After a preliminary regression using a 'good model', which satisfies reasonable goodness-of-fit criteria, the residuals were taken to represent the experimental error. The neighbouring residuals were grouped together and the sum of their mean squared values was used as a measure of the variance in the neighbourhood of the corresponding measurements. The values of the constants obtained in this way agreed with those obtained by replicates.
酶促稳态动力学实验的实验方差取决于速度，其近似为幂函数（Var(v) = K1·vα （阿斯克洛夫，P.，科斯费尔特，M. 和曼内维克，B.（1976年）《欧洲生物化学杂志》69卷，61 - 67页））。常数（K1，α）的值可通过对速度进行重复测量来估计，然后该函数的倒数可作为加权因子。为避免通过大量重复测量来确定动力学数据集的误差结构，测试了一种不同的方法。在使用满足合理拟合优度标准的“良好模型”进行初步回归后，取残差来表示实验误差。将相邻残差分组在一起，其均方值之和用作相应测量值邻域内方差的度量。以这种方式获得的常数的值与通过重复测量获得的值一致。
