Abuchowski A, Davis F F
Biochim Biophys Acta. 1979 May 23;578(1):41-6. doi: 10.1016/0005-2795(79)90110-7.
The covalent attachment of polyethylene glycol of 5000 daltons to non-essential groups on trypsin produces an adduct that no longer precipitates with anti-trypsin antibody. In comparison with trypsin, polyethylene glycol-trypsin preparations show equal or greater activity against N-alpha-benzoyl-L-arginine ethyl ester, about one-fourth activity against angiotensin II, and little activity against bovine liver catalase. The polyethylene glycol-trypsin adduct dissolves soft blood clots at one-fourth the rate of trypsin. Soybean trypsin inhibitor produces two-thirds inhibition of the adduct under conditions that cause complete inhibition of trypsin.
将5000道尔顿的聚乙二醇共价连接到胰蛋白酶的非必需基团上，会产生一种加合物，该加合物不再与抗胰蛋白酶抗体沉淀。与胰蛋白酶相比，聚乙二醇 - 胰蛋白酶制剂对N-α-苯甲酰-L-精氨酸乙酯显示出相同或更高的活性，对血管紧张素II的活性约为其四分之一，而对牛肝过氧化氢酶几乎没有活性。聚乙二醇 - 胰蛋白酶加合物溶解软血凝块的速度是胰蛋白酶的四分之一。在导致胰蛋白酶完全抑制的条件下，大豆胰蛋白酶抑制剂对该加合物产生三分之二的抑制作用。
