Fontes M, Railhac C, Thouveny Y
Biochimie. 1979;61(3):369-77. doi: 10.1016/s0300-9084(79)80130-3.
The binding of [3H] Uridine labelled RNAs and [32P] RNAs to poly U filters using the Sheldon et al. technique (1972) shows an increase in the poly A+ RNA populations from total RNAs and cytoplasmic RNAs in the prereplicative phase of the regenerating process in Owenia fusiformis. This increase confirms that the first stage of this process consists in an activation of the transcription of the genetic material concerning mainly mRNAs synthesis activation.
使用谢尔登等人(1972年)的技术,将[3H]尿苷标记的RNA和[32P]RNA与聚尿苷滤膜进行结合实验,结果显示,在纺锤欧文蛤再生过程的复制前期,总RNA和细胞质RNA中的多聚腺苷酸加尾(poly A+)RNA群体有所增加。这一增加证实了该过程的第一阶段主要在于激活与mRNA合成激活相关的遗传物质转录。