Chestukhina G G, Kotova T S, Zalunin I A, Stepanov V M
Biokhimiia. 1979 May;44(5):796-802.
Some serine proteases and leucine aminopeptidases were detected inside and outside the cells during the analysis of three crystalline and two acrystalline strains of Bac. thuringiensis var. galleriae. The data obtained on the protease formation during growth and sporulation and the level of their activity are indicative of intracellular proteases involvement in spore- and crystal formation. The enzymes isolated from the culture medium do not probably take part in these processes. The intracellular enzymes may account for the different crystal protein composition of various strains due to limited proteolysis of crystal proteins in the course of biosynthesis.
在对苏云金芽孢杆菌蜡螟变种的三个晶体菌株和两个无晶体菌株进行分析的过程中,在细胞内外均检测到了一些丝氨酸蛋白酶和亮氨酸氨肽酶。在生长和孢子形成过程中获得的有关蛋白酶形成的数据及其活性水平表明,细胞内蛋白酶参与了孢子和晶体的形成。从培养基中分离出的酶可能不参与这些过程。由于在生物合成过程中晶体蛋白的有限蛋白水解,细胞内酶可能是造成各菌株晶体蛋白组成不同的原因。