Eidus L, Varughese P, Hodgkin M M, Hsu A H, McRae K B
Bull World Health Organ. 1973;49(5):507-16.
In this investigation a simple urine test for phenotyping isoniazid inactivators is evaluated. In the new method, isoniazid is artificially acetylated in urine and determined by the same colour reaction as that used for acetylisoniazid. Comparative studies showed that the test is reliable and can be performed with accuracy and ease even in poorly equipped laboratories. In contrast to other urine tests, it does not require an expensive spectrophotometer, tedious hydrolysis processing of the samples, or standard curves. The results can be read on a plain colorimeter, or even without any instrument.
在本研究中，对一种用于异烟肼灭活剂表型分析的简单尿液检测方法进行了评估。在新方法中，异烟肼在尿液中被人工乙酰化，并通过与用于乙酰异烟肼相同的显色反应进行测定。对比研究表明，该检测方法可靠，即使在设备简陋的实验室也能准确、轻松地进行。与其他尿液检测方法不同，它不需要昂贵的分光光度计、繁琐的样品水解处理或标准曲线。结果可以在普通比色计上读取，甚至无需任何仪器。
