Ho D T, Varner J E
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4783-6. doi: 10.1073/pnas.71.12.4783.
Ribonucleic acid containing poly(adenylic acid) [poly(A)-RNA] is present in barley aleurone layers. This poly(A)-RNA becomes labeled with radioactive precursors of RNA during the incubation of isolated aleurone layers with or without gibberellic acid. However, the rate of synthesis of poly(A)-RNA is enhanced by gibberellic acid. This enhancement begins within 3-4 hr of addition of the hormone and reaches a maximum, which is about 50-60% over the control, 10-12 hr after addition of the hormone. Cordycepin inhibits total RNA as well as poly(A)-RNA synthesis in barley aleurone layers. However, cordycepin inhibits the hormone-controlled synthesis of alpha-amylase (EC 3.2.1.1) only if it is added 12 hr or less after gibberellic acid. The insensitivity of alpha-amylase production to cordycepin after 12 hr of gibberellic acid treatment suggests that alpha-amylase is translated from stable messenger RNA.
含聚腺苷酸的核糖核酸[聚(A)-RNA]存在于大麦糊粉层中。在离体糊粉层与赤霉素一起或不与赤霉素一起孵育期间,这种聚(A)-RNA会被RNA的放射性前体标记。然而,赤霉素可提高聚(A)-RNA的合成速率。这种提高在添加激素后的3 - 4小时内开始,并在添加激素后的10 - 12小时达到最大值,比对照高约50 - 60%。虫草素抑制大麦糊粉层中的总RNA以及聚(A)-RNA的合成。然而,虫草素仅在赤霉素添加后12小时或更短时间添加时,才会抑制激素控制的α-淀粉酶(EC 3.2.1.1)的合成。赤霉素处理12小时后α-淀粉酶产生对虫草素不敏感,这表明α-淀粉酶是由稳定的信使RNA翻译而来。