Jacobsen J V, Zwar J A
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3290-3. doi: 10.1073/pnas.71.8.3290.
Incubation of isolated barley aleurone layers with gibberellic acid for 16 hr caused a 50% increase in the synthesis of RNA that contains poly(A) sequences [poly(A)-RNA], but had no measurable effect on the syntheses of the major RNA species. The syntheses of both the poly(A) and the heteropolymeric fractions of the poly(A)-RNA were increased. The poly(A) sequences were separated into two classes by size, one containing an average of 250 nucleotides and the other about 70 nucleotides. The two classes occurred in a molar ratio of about 1:1. Gibberellic acid increased the syntheses of both sequences to the same extent. We interpret these results to mean that gibberellic acid increases specifically the synthesis of mRNA in this tissue.
将分离的大麦糊粉层与赤霉素一起温育16小时，导致含有聚腺苷酸序列的RNA（聚腺苷酸RNA）的合成增加了50%，但对主要RNA种类的合成没有可测量的影响。聚腺苷酸RNA的聚腺苷酸部分和杂聚部分的合成均增加。聚腺苷酸序列按大小分为两类，一类平均含有250个核苷酸，另一类约70个核苷酸。这两类的摩尔比约为1:1。赤霉素使这两种序列的合成增加到相同程度。我们将这些结果解释为赤霉素特异性地增加了该组织中mRNA的合成。
