MSU/AEC Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48823.
Plant Physiol. 1967 Mar;42(3):398-406. doi: 10.1104/pp.42.3.398.
Gibberellic acid enhances the synthesis of alpha-amylase in isolated aleurone layers of barley-seeds (Hordeum vulgare var. Himalaya). In the presence of 20 mm calcium chloride the amount of enzyme obtained from isolated aleurone layers is quantitatively comparable to that of the half-seeds used in earlier studies. After a lag period of 6 to 8 hours enzyme is produced at a linear rate. Gibberellic acid does not merely trigger alpha-amylase synthesis, but it is continuously required during the period of enzyme formation. Enzyme synthesis is inhibited by inhibitors of protein and RNA synthesis. Small amounts of actinomycin D differentially inhibit enzyme release and enzyme synthesis suggesting 2 distinct processes. Gibberellic acid similarly enhances the formation of ribonuclease which increases linearly over a 48 hour period. During the first 24 hours the enzyme is retained by the aleurone cells and this is followed by a rapid release of ribonuclease during the next 24 hour period. The capacity to release the enzyme is generated between 20 and 28 hours after the addition of the hormone. Ribonuclease formation is inhibited by inhibitors of protein and RNA synthesis. These inhibitors also prevent the formation of the release mechanism if added at the appropriate moment.
赤霉素可促进大麦（Hordeum vulgare var. Himalaya）糊粉层中α-淀粉酶的合成。在 20mM 氯化钙存在的情况下，从分离的糊粉层中获得的酶的数量与早期研究中使用的半粒种子的数量相当。经过 6 到 8 小时的潜伏期，酶以线性速率产生。赤霉素不仅触发α-淀粉酶的合成，而且在酶形成期间持续需要。蛋白质和 RNA 合成抑制剂抑制酶的合成。小剂量放线菌素 D 可差异抑制酶的释放和合成，表明存在 2 个不同的过程。赤霉素同样可增强核糖核酸酶的形成，在 48 小时内呈线性增加。在最初的 24 小时内，酶被糊粉细胞保留，随后在接下来的 24 小时内迅速释放核糖核酸酶。在添加激素后 20 到 28 小时之间产生释放酶的能力。核糖核酸酶的形成受蛋白质和 RNA 合成抑制剂的抑制。如果在适当的时间添加这些抑制剂，它们还会阻止释放机制的形成。
