Schur P H, DeAngelis D, Jackson J M
Clin Exp Immunol. 1974 May;17(1):209-18.
A counterimmunoelectrophoresis (CIEP) technique has been developed for the rapid, simple, specific detection of nucleic acids as antigens, or for the detection of precipitating antibodies to nucleic acids or nuclear antigens. The majority of precipitins could be detected within 1 hr. As little as 0·0015 μg of antigen per ml (e.g. poly A: poly U) could be detected. Specificity of rabbit antisera to nucleic acids was demonstrated by selective reactions using a panel of polynucleotides. 1091 patient sera were examined for precipitins to DNA, single-stranded DNA, nucleoprotein and calf thymus nucleoprotein. Precipitins to DNA were found in 42% of systemic lupus erythematosus sera, 9% of rheumatoid arthritis sera and 4% of juvenile rheumatoid arthritis sera. Results with the CIEP method showed equal sensitivity as results obtained by complement fixation or binding assays, but were more sensitive than double diffusion in agar (Ouchterlony).
已开发出一种对流免疫电泳（CIEP）技术，用于快速、简单、特异性地检测作为抗原的核酸，或检测针对核酸或核抗原的沉淀抗体。大多数沉淀素可在1小时内检测到。每毫升可检测到低至0.0015微克的抗原（例如聚腺苷酸：聚尿苷酸）。通过使用一组多核苷酸的选择性反应证明了兔抗核酸血清的特异性。对1091份患者血清检测了针对DNA、单链DNA、核蛋白和小牛胸腺核蛋白的沉淀素。在42%的系统性红斑狼疮血清、9%的类风湿性关节炎血清和4%的青少年类风湿性关节炎血清中发现了针对DNA的沉淀素。CIEP方法的结果显示出与补体结合或结合试验所获结果相同的灵敏度，但比琼脂双向扩散（欧氏）法更灵敏。
