辐射对培养的T淋巴细胞系的体外优先效应。
In vitro preferential effect of irradiation on cultured T lymphoid cell line.
作者信息
Han T, Pauly J L, Minowada J
出版信息
Clin Exp Immunol. 1974 Jul;17(3):455-62.
The present report describes a comparative study of cytocidal effect of irradiation on human cultured T and B lymphoid cells. Cytocidal effect of irradiation was expressed as percentage inhibition or percentage reduction, which was calculated by comparing the [H]thymidine incorporations or viable cell counts in irradiated and unirradiated cultures, respectively. A higher percentage inhibition was observed in each and every T-cell culture, irradiated with 100–4000 rads at days 1–4 of incubation. The most significant difference of percentage inhibition between T and B cells (<0·05–<0·01) was observed at each irradiation dose level at day 3. A significant percentage reduction of viable T cells (98–100%) was noted at 100 rad dose level, whereas only 0–3% of B lymphoid cells were killed by this dose of irradiation at days 2–4 of incubation. Our data clearly indicate that human cultured T lymphoid cells are extremely radiosensitive and B lymphoid cells, on the other hand, are fairly radioresistant.
本报告描述了辐射对人培养的T和B淋巴细胞的细胞杀伤作用的比较研究。辐射的细胞杀伤作用以抑制百分比或减少百分比表示，分别通过比较照射和未照射培养物中的[H]胸腺嘧啶核苷掺入量或活细胞计数来计算。在培养第1 - 4天用100 - 4000拉德照射的每一种T细胞培养物中，均观察到较高的抑制百分比。在第3天的每个辐射剂量水平下，T细胞和B细胞之间的抑制百分比差异最为显著（<0.05 - <0.01）。在100拉德剂量水平下，观察到活T细胞显著减少（98 - 100%），而在培养第2 - 4天，此剂量的辐射仅杀死0 - 3%的B淋巴细胞。我们的数据清楚地表明，人培养的T淋巴细胞对辐射极其敏感，而B淋巴细胞则相当抗辐射。
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