Klemm N, Murrin L C, Kuhar M J
Brain Res. 1979 Jun 15;169(1):1-9. doi: 10.1016/0006-8993(79)90369-x.
Rats were administered [3H]spiperone (SP: spiroperidol) by tail vein injection and 2 h later the brain was processed for light microscopic autoradiography. High densities of autoradiographic grains were found in all areas known to have a dopaminergic innervation, including the olfactory tubercles, nucleus accumbens, nucleus caudate-putamen, lateral septum, zona incerta, nucleus subthalamicus, arcuate nucleus, nucleus of the central amygdala, areas in the ventral tegmentum and the claustrum. There were also increased autoradiographic grain densities in other areas such as the midbrain and the frontal cortex indicating that binding occurred to other neurotransmitter receptors besides dopamine receptors. These studies delineate with a high resolution at an anatomical level the major binding sites for neuroleptic drugs in the forebrain. They suggest which areas of the brain are the most involved in neuroleptic drug action and they add further evidence that important regions are those receiving a dense dopaminergic innervation.
通过尾静脉注射给大鼠施用[3H]螺哌隆（SP：螺哌啶醇），2小时后对大脑进行处理以进行光学显微镜放射自显影。在所有已知有多巴胺能神经支配的区域发现了高密度的放射自显影颗粒，包括嗅结节、伏隔核、尾状核-壳核、外侧隔、未定带、丘脑底核、弓状核、中央杏仁核核、腹侧被盖区和屏状核。在中脑和额叶皮质等其他区域也有放射自显影颗粒密度增加，表明除多巴胺受体外，还与其他神经递质受体发生了结合。这些研究在解剖学水平上以高分辨率描绘了前脑中抗精神病药物的主要结合位点。它们表明大脑的哪些区域与抗精神病药物作用最相关，并且进一步证明重要区域是那些接受密集多巴胺能神经支配的区域。
