Myher J J, Kuksis A
Can J Biochem. 1979 Feb;57(2):117-24. doi: 10.1139/o79-015.
A method of simultaneous determination of stereospecific distribution and molecular association of acyl groups in triacylglycerols has been developed. The analysis is based on a random generation of rac-1,2-diacylglycerols by Grignard degradation, synthesis of rac-phosphatidylcholines, and a stereospecific stepwise release of 1,2-sn- and 2,3-sn-diacylglycerols by phospholipase C. The exact structure of the original triacylglycerols is reconstituted on the basis of complete analysis of the molecular species of the 1,2-sn- and 2,3-sn-diacylglycerols as the tertiary-butyldimethylsilyl ethers by gas chromatography with mass spectrometry. The validity of the method is demonstrated by analyses of synthetic triacylglycerols of known structure. A practical application is illustrated by determination of the fatty acid distribution in lard.
已开发出一种同时测定三酰甘油中酰基的立体特异性分布和分子缔合的方法。该分析基于通过格氏降解随机生成外消旋1,2-二酰甘油、合成外消旋磷脂酰胆碱，以及通过磷脂酶C立体特异性逐步释放1,2-sn-和2,3-sn-二酰甘油。通过气相色谱-质谱联用对作为叔丁基二甲基甲硅烷基醚的1,2-sn-和2,3-sn-二酰甘油的分子种类进行全面分析，从而重构原始三酰甘油的确切结构。通过对已知结构的合成三酰甘油进行分析，证明了该方法的有效性。通过测定猪油中的脂肪酸分布说明了该方法的实际应用。
