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World J Microbiol Biotechnol. 1995 Nov;11(6):669-77. doi: 10.1007/BF00361014.
Three extracellular lipases were produced by batch fermentation of Pseudomonas putida ATCC 795, Aspergillus niger CBS 131.52 and Rhizopus oryzae ATCC 34612 during the late phase of growth, at 72, 96 and 96 h, respectively. The lipases were partially purified by (NH4)2SO4 fractionation. The lipase of P. putida was optimal at pH 8.0 whereas those from A. niger and R. oryzae were optimal at pH 7.5. The A. niger lipase had the lowest V max value (0.51×10(-3) U/min) and R. oryzae the highest (1.86×10(-3) U/min). The K m values for P. putida, A. niger and R. oryzae lipases were 1.18, 0.97, and 0.98 mg/ml, respectively. Native PAGE of the partially-purified lipase extracts showed two to four major bands. The interesterification of butter fat by A. niger lipase decreased the water activity as well as the hydrolytic activity. The A. niger lipase had the highest interesterification yield value (26%) and the R. oryzae lipase the lowest (4%). In addition, A. niger lipase exhibited the highest decrease (17%) in long-chain hypercholesterolemic fatty acids (C12:0, C14:0 and C16:0) at the sn-2-position; the P. putida lipase demonstrated the least favourable changes in specificity at the same position.
三种胞外脂肪酶分别由铜绿假单胞菌 ATCC 795、黑曲霉 CBS 131.52 和米根霉 ATCC 34612 在生长后期的 72、96 和 96 小时分批发酵生产。脂肪酶经（NH4）2SO4 分级沉淀部分纯化。铜绿假单胞菌脂肪酶的最适 pH 值为 8.0，而黑曲霉和米根霉脂肪酶的最适 pH 值为 7.5。黑曲霉脂肪酶的 V max 值最低（0.51×10(-3) U/min），米根霉脂肪酶的 V max 值最高（1.86×10(-3) U/min）。铜绿假单胞菌、黑曲霉和米根霉脂肪酶的 K m 值分别为 1.18、0.97 和 0.98mg/ml。部分纯化的脂肪酶提取物的 native PAGE 显示出两到四条主要条带。黑曲霉脂肪酶对黄油脂肪的酯交换作用降低了水活度和水解活性。黑曲霉脂肪酶的酯交换产率最高（26%），米根霉脂肪酶最低（4%）。此外，黑曲霉脂肪酶在 sn-2 位对长链高胆固醇脂肪酸（C12:0、C14:0 和 C16:0）的减少量最大（17%）；而铜绿假单胞菌脂肪酶在同一位置的特异性变化最小。
