大肠杆菌素E3在体外对核糖体的失活作用及其作用机制。
Inactivation of ribosomes in vitro by colicin E 3 and its mechanism of action.
作者信息
Boon T
出版信息
Proc Natl Acad Sci U S A. 1972 Mar;69(3):549-52. doi: 10.1073/pnas.69.3.549.
Abstract
The incubation of purified ribosomes with colicin E(3) results in the cleavage of a terminal fragment from the 16S ribosomal RNA. The cleavage reaction requires three components: colicin E(3), the 30S ribosomal subunit, and the 50S ribosomal subunit. An immunity factor found in extracts derived from colicinogenic cells prevents the in vitro inactivation of ribosomes by colicin E(3). Evidence is presented suggesting that it does so by binding to the colicin molecule. The mode of action of colicin E(3)in vivo can be explained by the assumption that a small fraction of the adsorbed colicin penetrates into the cell and catalytically inactivates the ribosomes.
摘要
纯化的核糖体与大肠菌素E(3)温育会导致16S核糖体RNA的一个末端片段被切割。切割反应需要三个组分：大肠菌素E(3)、30S核糖体亚基和50S核糖体亚基。在产大肠菌素细胞提取物中发现的一种免疫因子可防止大肠菌素E(3)在体外使核糖体失活。有证据表明它是通过与大肠菌素分子结合来实现这一点的。大肠菌素E(3)在体内的作用方式可以通过以下假设来解释：一小部分吸附的大肠菌素穿透细胞并催化使核糖体失活。
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