大肠杆菌素E3在体外使核糖体失活。
Inactivation of ribosomes in vitro by colicin E 3 .
作者信息
Boon T
出版信息
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2421-5. doi: 10.1073/pnas.68.10.2421.
Abstract
Cell-free protein synthesizing extracts incubated with purified preparations of colicin E(3) showed a marked decrease of their protein synthesizing activity. Incubation of ribosomes prepared from S-30 extracts with E(3) resulted in their inactivation. Their 16S RNA was found to lose a terminal fragment, similar in size to the fragment released upon in vivo treatment with E(3). Extracts derived from colicinogenic strains are resistant to the action of E(3)in vitro.
摘要
与纯化的大肠杆菌素E(3)制剂一起孵育的无细胞蛋白质合成提取物显示出其蛋白质合成活性显著下降。用E(3)处理从S-30提取物制备的核糖体，导致其失活。发现它们的16S RNA失去了一个末端片段，其大小与体内用E(3)处理后释放的片段相似。来自产大肠杆菌素菌株的提取物在体外对E(3)的作用具有抗性。
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Interaction of colicins with bacterial cells. II. Specific alteration of Escherichia coli ribosomes induced by colicin E3 in vivo.大肠杆菌素与细菌细胞的相互作用。II. 体内大肠杆菌素E3诱导的大肠杆菌核糖体的特异性改变。
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Interaction of colicins with bacterial cells. I. Studies with radioactive colicins.大肠杆菌素与细菌细胞的相互作用。I. 放射性大肠杆菌素的研究。
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