一种用于测定大鼠肝脏和脑组织粗酶制剂中半胱磺酸脱羧酶活性的灵敏放射性测定法。
A sensitive radiometric assay for cysteic acid decarboxylase activity in crude enzyme preparations of rat liver and brain.
作者信息
Yam C F, Tunnicliff G, Ngo T T, Barbeau A
机构信息
Clinical Research Institute of Montreal, Quebec, Canada.
出版信息
Comp Biochem Physiol B. 1977;58(1):115-7. doi: 10.1016/0305-0491(77)90136-5.
PMID:45523
Abstract
- A method is described for the synthesis of L-[U-14C]cysteic acid from L-[U-14C] cysteine hydrochloride and for its subsequent utilisation as a substrate for cysteic acid decarboxylase activity in liver and brain. 2. The enzyme determination relies on the entrapment of radio-labelled carbon dioxide in Hyamine hydroxide. 3. The assay is sensitive, reliable and convenient and is particularly suitable for measuring the activity of the decarboxylase in crude enzyme preparations.
摘要
- 描述了一种从L-[U-¹⁴C]半胱氨酸盐酸盐合成L-[U-¹⁴C]半胱磺酸的方法，以及随后将其用作肝脏和大脑中半胱磺酸脱羧酶活性底物的用途。2. 酶的测定依赖于放射性标记的二氧化碳被氢氧化季铵捕获。3. 该测定方法灵敏、可靠且方便，特别适用于测定粗酶制剂中脱羧酶的活性。
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