Datta S C
Indian Institute of Chemical Biology, Jadavpur, Calcutta.
J Neurochem. 1988 Apr;50(4):999-1002. doi: 10.1111/j.1471-4159.1988.tb10564.x.
The possibilities of interference by glycerophosphoryl ethanolamine (GPE) in the estimation of taurine levels in cerebral cortex, midbrain, cerebellum, medullapons, and spinal cord of developing human fetal brain regions were eliminated by hydrolyzing tissue extracts with 6 M HCl. Cysteic acid thus produced was separated from taurine by ion-exchange chromatography using Biorad-AG resin. Fluorescamine was used as fluorogen. Data reveal that the estimation of taurine in human fetal brain regions is affected if GPE is present as a contaminant in the assay system. Cysteic acid decarboxylase activity was measured using cysteic acid as the substrate. Higher enzymic activity was recorded with increased fetal body weight, but the reverse was true for taurine level.
通过用6 M盐酸水解组织提取物，消除了甘油磷酰乙醇胺（GPE）对发育中的人类胎儿脑区大脑皮质、中脑、小脑、延髓和脊髓中牛磺酸水平估计的干扰可能性。使用Biorad-AG树脂通过离子交换色谱法将由此产生的半胱氨酸与牛磺酸分离。荧光胺用作荧光剂。数据表明，如果测定系统中存在GPE污染物，会影响人类胎儿脑区中牛磺酸的估计。以半胱氨酸为底物测量半胱氨酸脱羧酶活性。随着胎儿体重增加，酶活性记录更高，但牛磺酸水平则相反。
