Tsukamoto K, Sugino Y
J Virol. 1972 Mar;9(3):465-73. doi: 10.1128/JVI.9.3.465-473.1972.
Bovine adenovirus type 3 (BAV-3), which has been reported to produce tumors in newborn hamsters, induced cellular deoxyribonucleic acid (DNA) synthesis in a contact-inhibited mouse kidney cell line (C3H2K). In this system, the virus did not multiply, whereas virus-specific tumor antigen (T antigen) was detected in nearly all cells. Replication of viral DNA could not be detected by DNA-DNA hybridization on membrane filters. The cellular DNA synthesis induced by BAV-3 did occur in the absence of added serum. Extent of induction of cellular DNA synthesis was closely correlated with the multiplicity of infection. Cells activated to synthesize DNA in the serum-free medium by the virus infection progressed to cell division without noticeable cell killing.
据报道，牛3型腺病毒（BAV - 3）可在新生仓鼠体内诱发肿瘤，它能在接触抑制的小鼠肾细胞系（C3H2K）中诱导细胞脱氧核糖核酸（DNA）合成。在该系统中，病毒并不增殖，然而几乎在所有细胞中都能检测到病毒特异性肿瘤抗原（T抗原）。通过膜滤器上的DNA - DNA杂交无法检测到病毒DNA的复制。BAV - 3诱导的细胞DNA合成在未添加血清的情况下确实会发生。细胞DNA合成的诱导程度与感染复数密切相关。因病毒感染而在无血清培养基中被激活以合成DNA的细胞会进入细胞分裂阶段，且未观察到明显的细胞死亡现象。
