Reddy P S, Idamakanti N, Zakhartchouk A N, Baxi M K, Lee J B, Pyne C, Babiuk L A, Tikoo S K
Virology Group, Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
J Virol. 1998 Feb;72(2):1394-402. doi: 10.1128/JVI.72.2.1394-1402.1998.
The complete DNA sequence of bovine adenovirus type 3 is reported here. The size of the genome is 34,446 bp in length with a G+C content of 54%. All the genes of the early and late regions are present in the expected locations of the genome. However, the late-region genes are organized into seven families, instead of five as they are in human adenovirus type 2. The deduced amino acid sequences of open reading frames (ORFs) in the late regions and early region 2 (E2) and for IVa2 show higher degrees of homology, whereas the predicted amino acid sequences of ORFs in the E1, E3, and E4 regions and the pIX, fiber, and 33,000-molecular-weight nonstructural proteins show little or no homology with the corresponding proteins of other adenoviruses. In addition, the penton base protein lacks the integrin binding motif, RGD, but has an LDV motif instead of an MDV motif. Interestingly, as in other animal adenoviruses, the virus-associated RNA genes appear to be absent from their usual location. Sequence analysis of cDNA clones representing the early- and late-region genes identified splice acceptor and splice donor sites, polyadenylation signals and polyadenylation sites, and tripartite leader sequences.
本文报道了牛3型腺病毒的完整DNA序列。基因组大小为34446 bp，G+C含量为54%。早期和晚期区域的所有基因均位于基因组的预期位置。然而，晚期区域的基因被组织成七个家族，而不是像人2型腺病毒那样为五个家族。晚期区域和早期区域2（E2）以及IVa2的开放阅读框（ORF）推导的氨基酸序列显示出更高程度的同源性，而E1、E3和E4区域以及pIX、纤维和33000分子量非结构蛋白的ORF预测氨基酸序列与其他腺病毒的相应蛋白几乎没有同源性或无同源性。此外，五邻体基底蛋白缺乏整合素结合基序RGD，但具有LDV基序而非MDV基序。有趣的是，与其他动物腺病毒一样，病毒相关RNA基因似乎不在其通常位置。对代表早期和晚期区域基因的cDNA克隆进行序列分析，确定了剪接受体和剪接供体位点、聚腺苷酸化信号和聚腺苷酸化位点以及三联前导序列。
