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Phosphonoglycoprotein from Metridium senile--heterogeneity of glycoproteins containing aminoethylphosphonic acid.

作者信息

Hurley J C, Bunde T A, Dell J C, Kirkpatrick D S, Bishop S H

机构信息

Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.

出版信息

Comp Biochem Physiol B. 1977;58(3):253-9. doi: 10.1016/0305-0491(77)90199-7.

DOI:10.1016/0305-0491(77)90199-7
PMID:45526
Abstract
  1. After separation by SDS gel-chromatography, analysis of AEP-containing glycoproteins from M. senile, indicated 66% amino acids with 220 AEP res./1000 res. and 30% carbohydrate for high mol. wt (greater than 10(7) forms and 80% amino acids with 25-50 AEP res./1000 res. and 10% carbohydrate for low mol. wt (2-4 x 10(4) forms. 2. Uronic acids, sulfate, lipid, and sialic acids were absent. 3. Mild base digestion released AEP-hexosamine containing oligosaccharides and destroyed ser-thr residues in the high mol. wt components. 4. Phosphonoglycoproteins appear to be acidic connective tissue components with AEP linked to hexosamine containing oligosaccharide side chains.
摘要

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