Cook A M, Daughton C G, Alexander M
J Bacteriol. 1978 Jan;133(1):85-90. doi: 10.1128/jb.133.1.85-90.1978.
Bacteria able to use at least one of 13 ionic alkylphosphonates of O-alkyl or O,O-dialkyl alkylphosphonates as phosphorus sources were isolated from sewage and soil. Four of these isolates used 2-aminoethylphosphonic acid (AEP) as a sole carbon, nitrogen, and phosphorus source. None of the other phosphonates served as a carbon source for the organisms. One isolate, identified as Pseudomonas putida, grew with AEP as its sole carbon, nitrogen, and phosphorus source and released nearly all of the organic phosphorus as orthophosphate and 72% of the AEP nitrogen as ammonium. This is the first demonstration of utilization of a phosphonoalkyl moiety as a sole carbon source. Cell-free extracts of P. putida contained an inducible enzyme system that required pyruvate and pyridoxal phosphate to release orthophosphate from AEP; acetaldehyde was tentatively identified as a second product. Phosphite inhibited the enzyme system.
从污水和土壤中分离出了能够利用13种O-烷基或O,O-二烷基烷基膦酸酯中的至少一种作为磷源的细菌。其中有4株分离菌能够利用2-氨基乙基膦酸（AEP）作为唯一的碳、氮和磷源。其他膦酸酯均不能作为这些微生物的碳源。其中一株被鉴定为恶臭假单胞菌的分离菌，能够以AEP作为唯一的碳、氮和磷源生长，并将几乎所有的有机磷以正磷酸盐的形式释放出来，同时将72%的AEP氮以铵的形式释放出来。这是首次证明膦酰基烷基部分可作为唯一碳源被利用。恶臭假单胞菌的无细胞提取物含有一种诱导酶系统，该系统需要丙酮酸和磷酸吡哆醛才能从AEP中释放出正磷酸盐；初步鉴定乙醛为第二种产物。亚磷酸盐抑制该酶系统。
