Price W R, Olsen R A, Hunter J E
Appl Microbiol. 1972 Apr;23(4):679-82. doi: 10.1128/am.23.4.679-682.1972.
A method has been described for testing multiple food samples for Salmonella without loss in sensitivity. The method pools multiple pre-enrichment broth cultures into single enrichment broths. The subsequent stages of the Salmonella analysis are not altered. The method was found applicable to several dry food materials including nonfat dry milk, dried egg albumin, cocoa, cottonseed flour, wheat flour, and shredded coconut. As many as 25 pre-enrichment broth cultures were pooled without apparent loss in the sensitivity of Salmonella detection as compared to individual sample analysis. The procedure offers a simple, yet effective, way to increase sample capacity in the Salmonella testing of foods, particularly where a large proportion of samples ordinarily is negative. It also permits small portions of pre-enrichment broth cultures to be retained for subsequent individual analysis if positive tests are found. Salmonella testing of pooled pre-enrichment broths provides increased consumer protection for a given amount of analytical effort as compared to individual sample analysis.
已描述了一种用于检测多种食品样本中沙门氏菌且不损失灵敏度的方法。该方法将多个预富集肉汤培养物合并到单个富集肉汤中。沙门氏菌分析的后续阶段不变。该方法被发现适用于几种干食品原料，包括脱脂奶粉、干蛋清、可可、棉籽粉、小麦粉和椰丝。与单个样本分析相比，多达25个预富集肉汤培养物合并后，沙门氏菌检测的灵敏度没有明显损失。该程序提供了一种简单而有效的方法来增加食品沙门氏菌检测中的样本量，特别是在大部分样本通常为阴性的情况下。如果检测结果为阳性，它还允许保留少量预富集肉汤培养物用于后续的个体分析。与单个样本分析相比，对合并的预富集肉汤进行沙门氏菌检测可为给定的分析工作量提供更高的消费者保护。
