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用于食品和环境拭子样本中沙门氏菌微生物学检测的直接荧光抗体技术。

Direct fluorescent-antibody technique for the microbiological examination of food and environmental swab samples for salmonellae.

作者信息

Insalata N F, Mahnke C W, Dunlap W G

出版信息

Appl Microbiol. 1973 Sep;26(3):268-70. doi: 10.1128/am.26.3.268-270.1973.

DOI:10.1128/am.26.3.268-270.1973
PMID:4584574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC379772/
Abstract

Comparative studies of a modified fluorescent-antibody procedure and the 5 to 7 day method used by the Association of Official Analytical Chemists for the detection of Salmonella were made on 151 samples of wheat products and 183 swab samples. The agreement between the two methods for the 334 samples tested was 92.5%. Food samples yielded 94.7% agreement, whereas the swab samples yielded 90.7% agreement. There were 7.5% false positives for the total number of samples tested. No false negatives were obtained by using the fluorescent-antibody method.

摘要

对151份小麦制品样本和183份拭子样本进行了改良荧光抗体检测程序与美国官方分析化学家协会用于检测沙门氏菌的5至7天检测方法的对比研究。对334份测试样本而言,两种方法的符合率为92.5%。食品样本的符合率为94.7%,而拭子样本的符合率为90.7%。在所检测的样本总数中,假阳性率为7.5%。使用荧光抗体法未出现假阴性。

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本文引用的文献

1
Immunofluorescence technique for the detection of salmonellae in various foods.用于检测各类食品中沙门氏菌的免疫荧光技术。
Appl Microbiol. 1967 Sep;15(5):1145-9. doi: 10.1128/am.15.5.1145-1149.1967.
2
DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY.用荧光抗体检测鸡蛋和蛋制品中的沙门氏菌
Appl Microbiol. 1964 Sep;12(5):447-50. doi: 10.1128/am.12.5.447-450.1964.
3
A RAPID IMMUNOFLUORESCENCE TECHNIQUE FOR DETECTING SALMONELLAE IN RAW MEAT.一种用于检测生肉中沙门氏菌的快速免疫荧光技术。
J Hyg (Lond). 1964 Sep;62(3):319-27. doi: 10.1017/s0022172400040031.
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Further evaluation of a rapid immunofluorescence technique for detecting salmonellae in meat and poultry.用于检测肉类和禽类中沙门氏菌的快速免疫荧光技术的进一步评估。
J Appl Bacteriol. 1965 Dec;28(3):421-5. doi: 10.1111/j.1365-2672.1965.tb02172.x.
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Preparation and testing of polyvalent conjugates for fluorescent-antibody detection of salmonellae.用于沙门氏菌荧光抗体检测的多价缀合物的制备与测试。
Appl Microbiol. 1971 Nov;22(5):876-84. doi: 10.1128/am.22.5.876-884.1971.
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Accelerated immunofluorescence procedure for the detection of Salmonella in foods and animal by-products.用于检测食品和动物副产品中沙门氏菌的快速免疫荧光程序。
Appl Microbiol. 1969 Nov;18(5):708-13. doi: 10.1128/am.18.5.708-713.1969.
7
Rapid, direct fluorescent-antibody method for the detection of salmonellae in food and feeds.用于检测食品和饲料中沙门氏菌的快速直接荧光抗体法。
Appl Microbiol. 1972 Oct;24(4):645-9. doi: 10.1128/am.24.4.645-649.1972.
8
Salmonella testing of pooled pre-enrichment broth cultures for screening multiple food samples.用于筛查多个食品样本的合并预增菌肉汤培养物的沙门氏菌检测。
Appl Microbiol. 1972 Apr;23(4):679-82. doi: 10.1128/am.23.4.679-682.1972.