用于食品和环境拭子样本中沙门氏菌微生物学检测的直接荧光抗体技术。
Direct fluorescent-antibody technique for the microbiological examination of food and environmental swab samples for salmonellae.
作者信息
Insalata N F, Mahnke C W, Dunlap W G
出版信息
Appl Microbiol. 1973 Sep;26(3):268-70. doi: 10.1128/am.26.3.268-270.1973.
Abstract
Comparative studies of a modified fluorescent-antibody procedure and the 5 to 7 day method used by the Association of Official Analytical Chemists for the detection of Salmonella were made on 151 samples of wheat products and 183 swab samples. The agreement between the two methods for the 334 samples tested was 92.5%. Food samples yielded 94.7% agreement, whereas the swab samples yielded 90.7% agreement. There were 7.5% false positives for the total number of samples tested. No false negatives were obtained by using the fluorescent-antibody method.
摘要
对151份小麦制品样本和183份拭子样本进行了改良荧光抗体检测程序与美国官方分析化学家协会用于检测沙门氏菌的5至7天检测方法的对比研究。对334份测试样本而言，两种方法的符合率为92.5%。食品样本的符合率为94.7%，而拭子样本的符合率为90.7%。在所检测的样本总数中，假阳性率为7.5%。使用荧光抗体法未出现假阴性。
相似文献
1
Direct fluorescent-antibody technique for the microbiological examination of food and environmental swab samples for salmonellae.
Appl Microbiol. 1973 Sep;26(3):268-70. doi: 10.1128/am.26.3.268-270.1973.
2
Evaluation of a fluorescent antibody-enrichment serology combination procedure for the detection of salmonellae in condiments, food products, food by-products, and animal feeds.
Appl Microbiol. 1973 Nov;26(5):751-6. doi: 10.1128/am.26.5.751-756.1973.
3
Comparison of fluorescent-antibody methods and enrichment serology for the detection of Salmonella.
Appl Microbiol. 1974 Feb;27(2):324-8. doi: 10.1128/am.27.2.324-328.1974.
4
Rapid, direct fluorescent-antibody method for the detection of salmonellae in food and feeds.
Appl Microbiol. 1972 Oct;24(4):645-9. doi: 10.1128/am.24.4.645-649.1972.
5
Evaluation of the salmonellae fluoro-kit for fluorescent-antibody staining.
Appl Microbiol. 1973 Feb;25(2):202-4. doi: 10.1128/am.25.2.202-204.1973.
6
One-day fluorescent-antibody procedure for detecting salmonellae in frozen and dried foods.
Appl Microbiol. 1970 Dec;20(6):977-83. doi: 10.1128/am.20.6.977-983.1970.
7
Evaluation of an automated fluorescent antibody procedure for detection of Salmonella in foods and feeds.
Appl Environ Microbiol. 1976 Apr;31(4):514-21. doi: 10.1128/aem.31.4.514-521.1976.
8
A rapid, presumptive procedure for the detection of Salmonella in foods and food ingredients.
Appl Microbiol. 1973 Jan;25(1):123-9. doi: 10.1128/am.25.1.123-129.1973.
9
Fluorescent-antibody technique in detection of salmonellae in animal feed and feed ingredients.
Appl Microbiol. 1969 Mar;17(3):352-4. doi: 10.1128/am.17.3.352-354.1969.
10
Accelerated immunofluorescence procedure for the detection of Salmonella in foods and animal by-products.
Appl Microbiol. 1969 Nov;18(5):708-13. doi: 10.1128/am.18.5.708-713.1969.
引用本文的文献
1
Detection of Salmonella serovars from clinical samples by enrichment broth cultivation-PCR procedure.
J Clin Microbiol. 1994 Jul;32(7):1742-9. doi: 10.1128/jcm.32.7.1742-1749.1994.
2
Rapid detection of Salmonella spp. in food by use of the ISO-GRID hydrophobic grid membrane filter.
Appl Environ Microbiol. 1982 Feb;43(2):261-8. doi: 10.1128/aem.43.2.261-268.1982.
本文引用的文献
1
Immunofluorescence technique for the detection of salmonellae in various foods.
Appl Microbiol. 1967 Sep;15(5):1145-9. doi: 10.1128/am.15.5.1145-1149.1967.
2
DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY.
Appl Microbiol. 1964 Sep;12(5):447-50. doi: 10.1128/am.12.5.447-450.1964.
3
A RAPID IMMUNOFLUORESCENCE TECHNIQUE FOR DETECTING SALMONELLAE IN RAW MEAT.
J Hyg (Lond). 1964 Sep;62(3):319-27. doi: 10.1017/s0022172400040031.
4
Further evaluation of a rapid immunofluorescence technique for detecting salmonellae in meat and poultry.
J Appl Bacteriol. 1965 Dec;28(3):421-5. doi: 10.1111/j.1365-2672.1965.tb02172.x.
5
Preparation and testing of polyvalent conjugates for fluorescent-antibody detection of salmonellae.
Appl Microbiol. 1971 Nov;22(5):876-84. doi: 10.1128/am.22.5.876-884.1971.
6
Accelerated immunofluorescence procedure for the detection of Salmonella in foods and animal by-products.
Appl Microbiol. 1969 Nov;18(5):708-13. doi: 10.1128/am.18.5.708-713.1969.
7
Rapid, direct fluorescent-antibody method for the detection of salmonellae in food and feeds.
Appl Microbiol. 1972 Oct;24(4):645-9. doi: 10.1128/am.24.4.645-649.1972.
8
Salmonella testing of pooled pre-enrichment broth cultures for screening multiple food samples.
Appl Microbiol. 1972 Apr;23(4):679-82. doi: 10.1128/am.23.4.679-682.1972.
Zotero 插件