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用于食品和环境拭子样本中沙门氏菌微生物学检测的直接荧光抗体技术。

Direct fluorescent-antibody technique for the microbiological examination of food and environmental swab samples for salmonellae.

作者信息

Insalata N F, Mahnke C W, Dunlap W G

出版信息

Appl Microbiol. 1973 Sep;26(3):268-70. doi: 10.1128/am.26.3.268-270.1973.

Abstract

Comparative studies of a modified fluorescent-antibody procedure and the 5 to 7 day method used by the Association of Official Analytical Chemists for the detection of Salmonella were made on 151 samples of wheat products and 183 swab samples. The agreement between the two methods for the 334 samples tested was 92.5%. Food samples yielded 94.7% agreement, whereas the swab samples yielded 90.7% agreement. There were 7.5% false positives for the total number of samples tested. No false negatives were obtained by using the fluorescent-antibody method.

摘要

对151份小麦制品样本和183份拭子样本进行了改良荧光抗体检测程序与美国官方分析化学家协会用于检测沙门氏菌的5至7天检测方法的对比研究。对334份测试样本而言,两种方法的符合率为92.5%。食品样本的符合率为94.7%,而拭子样本的符合率为90.7%。在所检测的样本总数中,假阳性率为7.5%。使用荧光抗体法未出现假阴性。

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