产气克雷伯菌1082E和阴沟肠杆菌P99的β-内酰胺酶底物特异性比较。
Comparison of the substrate specificities of the -lactamases from Klebsiella aerogenes 1082E and Enterobacter cloacae P99.
作者信息
Marshall M J, Ross G W, Chanter K V, Harris A M
出版信息
Appl Microbiol. 1972 Apr;23(4):765-9. doi: 10.1128/am.23.4.765-769.1972.
Abstract
A potent beta-lactamase (EC 3.5.2.6) produced by a strain of Klebsiella aerogenes (K. pneumoniae), 1082E, isolated from a hospital patient, has been examined. Its properties were different from those of most gram-negative beta-lactamases previously reported. The enzyme has been partly purified, and its activity against a range of substrates has been compared with that of the enzyme from Enterobacter cloacae (Aerobacter cloacae) P99. The K. aerogenes enzyme, although predominantly a penicillinase, had a wide range of specificity. In addition to hydrolyzing the cephalosporins, it attacked the normally beta-lactamaseresistant compounds methicillin and cloxacillin as well as cephalosporin analogues with the same acyl substituents. The results obtained with the E. cloacae enzyme confirmed its cephalosporinase activity and showed that, unlike the enzyme from K. aerogenes, it was relatively inactive against the penicillins.
摘要
对从一名医院患者身上分离出的产气克雷伯菌（肺炎克雷伯菌）1082E菌株产生的一种强效β-内酰胺酶（EC 3.5.2.6）进行了研究。其特性与先前报道的大多数革兰氏阴性β-内酰胺酶不同。该酶已部分纯化，并且已将其对一系列底物的活性与阴沟肠杆菌（产气气杆菌）P99产生的酶的活性进行了比较。产气克雷伯菌酶虽然主要是一种青霉素酶，但具有广泛的特异性。除了水解头孢菌素外，它还能攻击通常对β-内酰胺酶耐药的化合物甲氧西林和氯唑西林以及具有相同酰基取代基的头孢菌素类似物。用阴沟肠杆菌酶获得的结果证实了其头孢菌素酶活性，并表明，与产气克雷伯菌产生的酶不同，它对青霉素的活性相对较低。
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