λ噬菌体的大规模生产及纯化的λ脱氧核糖核酸
Large-scale production of lambda bacteriophage and purified lambda deoxyribonucleic acid.
作者信息
De Czekala A, Luk D, Bartl P
出版信息
Appl Microbiol. 1972 Apr;23(4):791-5. doi: 10.1128/am.23.4.791-795.1972.
Abstract
Large amounts of a heat-inducible phage lambda mutant (lambdaCI857) may be obtained under standardized conditions. The phage is harvested by simple polyethylene glycol (C 6000) precipitation and purified by CsCl density gradient banding. Deoxyribonucleic acid (DNA) is extracted by cold phenol and purified by sucrose density gradient sedimentation to yield a homogenous population of unbroken lambda DNA molecules.
摘要
在标准化条件下可获得大量热诱导型噬菌体λ突变体（λCI857）。通过简单的聚乙二醇（C 6000）沉淀收获噬菌体，并通过氯化铯密度梯度离心进行纯化。用冷酚提取脱氧核糖核酸（DNA），并通过蔗糖密度梯度沉降进行纯化，以产生均匀的完整λDNA分子群体。
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