噬菌体T1在气溶胶中的失活机制。
Mechanism of the inactivation of the bacteriophage T 1 in aerosols.
作者信息
Trouwborst T, de Jong J C
出版信息
Appl Microbiol. 1972 May;23(5):938-41. doi: 10.1128/am.23.5.938-941.1972.
Abstract
The mechanism of inactivation of bacteriophage T(1) in aerosols was studied by using (32)P-labeled phage. During inactivation, viability decreased in parallel with the adsorption of (32)P to the host, showing either that inactivated phage does not adsorb or that the deoxyribonucleic acid (DNA) has left the coat. A (32)P band at the density of free DNA was found when inactivated phage was analyzed in a CsCl gradient.
摘要
利用³²P标记的噬菌体研究了噬菌体T(1)在气溶胶中的失活机制。在失活过程中，活力的下降与³²P吸附到宿主上的情况平行，这表明要么失活的噬菌体不吸附，要么脱氧核糖核酸（DNA）已离开衣壳。当在CsCl梯度中分析失活的噬菌体时，发现了一条处于游离DNA密度的³²P带。
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