van der Rhee H J, van der Burgh-de Winter C P, Tijssen J G, Daems W T
Cell Tissue Res. 1979 Apr 12;197(3):397-412. doi: 10.1007/BF00233565.
Inflammatory reactions were evoked by simultaneous implantation of pieces of Melinex plastic in the subcutaneous tissue of the dorsum and in the peritoneal cavity of rats. The cellular composition of the Melinex-adherent cells and their peroxidatic (PO) activity were investigated in relation to the duration of implantation. Several striking differences were found between the subcutaneous and peritoneal implants. On the 7th and 14th days, multinucleated giant cells were abundantly present on the subcutaneous implants, whereas they were relatively rare on the peritoneal implants. The subcutaneous implants bore no mast cells and only a few eosinophilic granulocytes, but both types of cell were observed frequently on the peritoneal implants. Macrophages and multinucleated giant cells on the subcutaneous implants show PO activity only in the granules are PO negative. On the peritoneal implants three types of macrophages can be dintinguished: exudate macrophages which have PO activity restricted to granules or are PO-negative; macrophages with PO activity in granules and both the rough endoplasmic reticulum (RER) and nuclear envelope; and resident macrophages with PO activity only in the RER and nuclear envelope. In addition, two types of multinucleated giant cells are found, one with and the other without PO activity in the RER and nuclear envelope. Multinucleated giant cells with PO activity in the RER and nuclear envelope as well as exudate macrophages with PO activity in the RER and nuclear envelope were mainly found 32 h and 3 days after implantation of the Melinex in the peritoneal cavity. These findings are discussed in the light of current knowledge of the PO activity in macrophages and multinucleated giant cells. It is concluded that the appearance of PO activity in the RER and nuclear envelope of exudate macrophages and multinucleated giant cells is in all probability a transient phenomenon, and that there is no objective evidence to support the opinion that exudate macrophages with PO activity in the RER and nuclear envelope are transitional cells between exudate and resident macrophages.
通过在大鼠背部皮下组织和腹腔同时植入Melinex塑料片引发炎症反应。研究了与植入持续时间相关的Melinex黏附细胞的细胞组成及其过氧化物酶（PO）活性。皮下和腹腔植入物之间发现了几个显著差异。在第7天和第14天，皮下植入物上大量存在多核巨细胞，而在腹腔植入物上相对较少。皮下植入物上没有肥大细胞，只有少数嗜酸性粒细胞，但在腹腔植入物上这两种细胞都经常观察到。皮下植入物上的巨噬细胞和多核巨细胞仅在颗粒中显示PO活性，细胞核PO阴性。在腹腔植入物上可区分出三种类型的巨噬细胞：渗出性巨噬细胞，其PO活性仅限于颗粒或PO阴性；颗粒中有PO活性且粗面内质网（RER）和核膜均有PO活性的巨噬细胞；仅在RER和核膜中有PO活性的常驻巨噬细胞。此外，还发现了两种类型的多核巨细胞，一种在RER和核膜中有PO活性，另一种没有。在Melinex植入腹腔后32小时和3天，主要发现RER和核膜中有PO活性的多核巨细胞以及RER和核膜中有PO活性的渗出性巨噬细胞。根据目前对巨噬细胞和多核巨细胞中PO活性的了解对这些发现进行了讨论。得出的结论是，渗出性巨噬细胞和多核巨细胞的RER和核膜中PO活性的出现很可能是一种短暂现象，而且没有客观证据支持认为RER和核膜中有PO活性的渗出性巨噬细胞是渗出性巨噬细胞和常驻巨噬细胞之间过渡细胞的观点。
