伴刀豆球蛋白A对淋巴细胞的激活作用需要钙离子。

Activation of lymphocytes by concanavalin A requires calcium ions.

作者信息

Milner S M

出版信息

Cell Biol Int Rep. 1979 Jan;3(1):35-43. doi: 10.1016/0309-1651(79)90066-3.

Abstract

Mouse spleen cells were exposed to a short pulse of the mitogenic lectin concanavalin A (con A). After removal of con A mitogenesis was measured by the incorporation of tritiated thymidine into DNA. It was found: (a) the number of cells responding to con A was proportional to the time of exposure to con A; (b) exposure of cells to con A in the absence of extracellular calcium failed to initiate mitogenesis; (c) for a mitogenic effect an extracellular calcium concentration greater than 10(-5)M was required during the time that the cells were exposed to con A.

摘要

将小鼠脾细胞暴露于促有丝分裂凝集素伴刀豆球蛋白A（Con A）的短脉冲中。去除Con A后，通过将氚标记的胸腺嘧啶核苷掺入DNA来测量有丝分裂情况。结果发现：（a）对Con A作出反应的细胞数量与暴露于Con A的时间成正比；（b）在没有细胞外钙的情况下将细胞暴露于Con A无法启动有丝分裂；（c）为产生促有丝分裂效应，在细胞暴露于Con A的时间段内，细胞外钙浓度需要大于10⁻⁵M。