人红细胞血影膜插入颗粒的平移流动性。pH依赖性可逆聚集。
Translational mobility of the membrane intercalated particles of human erythrocyte ghosts. pH-dependent, reversible aggregation.
作者信息
Pinto da Silva P
出版信息
J Cell Biol. 1972 Jun;53(3):777-87. doi: 10.1083/jcb.53.3.777.
Abstract
This paper demonstrates the translational movement along the plane of the human erythrocyte ghost of the membrane particles exposed by freeze-fracture. The membrane particles can be aggregated by incubation of the ghosts in media with a pH in the vicinity of 5 5 or 3 5. The particles are disaggregated in neutral and alkaline media (pH 9 5) and also at pH 4.5 Aggregation of the particles at pH 5.5 is reversible, prevented by prefixation in glutaraldehyde and by media of high ionic strength. Particle aggregation occurs within 2-4 min. These results are consistent with the concept that the erythrocyte ghost membrane is a planar fluid domain formed by a bilayer membrane continuum which is interrupted by localized, yet mobile, proteic intercalations.
摘要
本文展示了通过冷冻断裂暴露的膜颗粒在人红细胞血影平面上的平移运动。通过将血影在pH值接近5.5或3.5的介质中孵育，膜颗粒可以聚集。在中性和碱性介质（pH 9.5）以及pH 4.5时，颗粒会解聚。在pH 5.5时颗粒的聚集是可逆的，戊二醛预固定和高离子强度介质可阻止这种聚集。颗粒聚集在2 - 4分钟内发生。这些结果与以下概念一致，即红细胞血影膜是由双层膜连续体形成的平面流体域，该连续体被局部但可移动的蛋白质插入物中断。
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