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1
Translational mobility of the membrane intercalated particles of human erythrocyte ghosts. pH-dependent, reversible aggregation.人红细胞血影膜插入颗粒的平移流动性。pH依赖性可逆聚集。
J Cell Biol. 1972 Jun;53(3):777-87. doi: 10.1083/jcb.53.3.777.
2
Membrane intercalated particles in human erythrocyte ghosts: sites of preferred passage of water molecules at low temperature.人红细胞血影中的膜嵌入颗粒:低温下水分子优先通过的部位。
Proc Natl Acad Sci U S A. 1973 May;70(5):1339-43. doi: 10.1073/pnas.70.5.1339.
3
Intramembrane particle aggregation in erythrocyte ghosts. I. The effects of protein removal.红细胞血影中的膜内颗粒聚集。I. 蛋白质去除的影响。
J Cell Biol. 1974 Dec;63(3):1018-36. doi: 10.1083/jcb.63.3.1018.
4
Intramembrane particle aggregation in erythrocyte ghosts. II. The influence of spectrin aggregation.红细胞血影中的膜内颗粒聚集。II. 血影蛋白聚集的影响。
Biochim Biophys Acta. 1976 Feb 19;426(1):101-22. doi: 10.1016/0005-2736(76)90433-8.
5
Anionic sites on the membrane intercalated particles of human erythrocyte ghost membranes. Freeze-etch localization.人红细胞血影膜膜间嵌入颗粒上的阴离子位点。冷冻蚀刻定位
Exp Cell Res. 1973 Sep;81(1):127-38. doi: 10.1016/0014-4827(73)90119-5.
6
Anionic sites of human erythrocyte membranes. I. Effects of trypsin, phospholipase C, and pH on the topography of bound positively charged colloidal particles.人红细胞膜的阴离子位点。I. 胰蛋白酶、磷脂酶C和pH对结合的带正电荷胶体颗粒拓扑结构的影响。
J Cell Biol. 1973 May;57(2):373-87. doi: 10.1083/jcb.57.2.373.
7
Freeze-etch localization of concanavalin A receptors to the membrane intercalated particles of human erythrocyte ghost membranes.伴刀豆球蛋白A受体在人红细胞血影膜的膜嵌入颗粒上的冷冻蚀刻定位。
Biochim Biophys Acta. 1974 Sep 23;363(3):311-9. doi: 10.1016/0005-2736(74)90071-6.
8
[The cleaving of membranes during freeze-etching demonstrated on erythrocyte ghosts and alterations of the membrane structure by treatment with urea].[在红细胞血影上展示的冷冻蚀刻过程中膜的裂解以及用尿素处理对膜结构的改变]
Protoplasma. 1970;70(2):233-46. doi: 10.1007/BF01276983.
9
Freeze-etching study on membrane ultrastructural changes caused by freezing: cooling rate-dependent intramembrane particle aggregation in erythrocyte stripped ghosts.关于冷冻引起的膜超微结构变化的冷冻蚀刻研究:红细胞脱脂影中膜内颗粒聚集与冷却速率有关。
Cryobiology. 1985 Feb;22(1):69-76. doi: 10.1016/0011-2240(85)90009-4.
10
Diminished spectrin extraction from ATP-depleted human erythrocytes. Evidence relating spectrin to changes in erythrocyte shape and deformability.从ATP耗竭的人红细胞中提取的血影蛋白减少。血影蛋白与红细胞形状和变形性变化相关的证据。
J Clin Invest. 1978 Mar;61(3):815-27. doi: 10.1172/JCI108996.

引用本文的文献

1
Cell physiology and molecular mechanism of anion transport by erythrocyte band 3/AE1.红细胞带 3/AE1 转运阴离子的细胞生理学和分子机制。
Am J Physiol Cell Physiol. 2021 Dec 1;321(6):C1028-C1059. doi: 10.1152/ajpcell.00275.2021. Epub 2021 Oct 20.
2
Cell-wall formation in Pelvetia embryos. A freeze-fracture study.海藻胚胎细胞壁的形成。冷冻断裂研究。
Planta. 1976 Jan;133(1):57-71. doi: 10.1007/BF00386007.
3
Cell contacts in the mouse mammary gland: i. Normal gland in postnatal development and the secretory cycle. 1973.小鼠乳腺中的细胞接触:i. 出生后发育及分泌周期中的正常腺体。1973年。
J Mammary Gland Biol Neoplasia. 2009 Sep;14(3):295-316. doi: 10.1007/s10911-009-9131-y.
4
Appearance and distribution of surface proteins of the human erythrocyte membrane. An electron microscope and immunochemical labeling study.人红细胞膜表面蛋白的外观与分布。一项电子显微镜及免疫化学标记研究。
J Cell Biol. 1978 Feb;76(2):512-31. doi: 10.1083/jcb.76.2.512.
5
Cytoplasmic pH and human erythrocyte shape.细胞质pH值与人类红细胞形态
Biophys J. 1997 Mar;72(3):1234-46. doi: 10.1016/S0006-3495(97)78770-8.
6
Electron microscopic study of reassociation of spectrin and actin with the human erythrocyte membrane.血影蛋白和肌动蛋白与人红细胞膜重新结合的电子显微镜研究。
J Cell Biol. 1981 Jul;90(1):70-7. doi: 10.1083/jcb.90.1.70.
7
Fine structure of intramembranous particle aggregates in ADH-treated frog urinary bladder and skin: influence of glutaraldehyde and N-ethyl maleimide.抗利尿激素处理的青蛙膀胱和皮肤中膜内颗粒聚集体的精细结构:戊二醛和N-乙基马来酰亚胺的影响
Cell Tissue Res. 1981;218(3):595-606. doi: 10.1007/BF00210118.
8
Evidence that ADH-stimulated intramembrane particle aggregates are transferred from cytoplasmic to luminal membranes in toad bladder epithelial cells.抗利尿激素刺激的膜内颗粒聚集体从蟾蜍膀胱上皮细胞的细胞质膜转移至腔面膜的证据。
J Cell Biol. 1980 Apr;85(1):83-95. doi: 10.1083/jcb.85.1.83.
9
Cell surface modifications in the epithelium of rat ventral prostate during adaptation to in vitro conditions: an ultrastructural study.大鼠腹侧前列腺上皮在适应体外条件过程中的细胞表面修饰:一项超微结构研究。
In Vitro. 1984 Mar;20(3 Pt 1):216-28. doi: 10.1007/BF02618190.
10
Ultrastructural alterations in the livers of golden hamsters following experimental chronic inhalation of diluted diesel exhaust emission.实验性长期吸入稀释的柴油机废气排放后金黄地鼠肝脏的超微结构改变
Int Arch Occup Environ Health. 1981;48(2):147-57. doi: 10.1007/BF00378435.

本文引用的文献

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Protein conformation in cell membrane preparations as studied by optical rotatory dispersion and circular dichroism.通过旋光色散和圆二色性研究细胞膜制剂中的蛋白质构象。
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1828-35. doi: 10.1073/pnas.56.6.1828.
2
CALCIUM AND MAGNESIUM BINDING PROPERTIES OF CELL MEMBRANE MATERIALS.细胞膜材料的钙镁结合特性
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SODIUM, POTASSIUM AND HYDROGEN ION BINDING CHARACTERISTICS OF DOG ERYTHROCYTE GHOSTS.犬红细胞血影的钠、钾和氢离子结合特性
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Purification by means of detergents and properties of cytochrome b5 from liver microsomes.利用去污剂从肝微粒体中纯化细胞色素b5及其性质
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6
Steady-state sieving across membranes.跨膜稳态筛分
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A major protein which spans the human erythrocyte membrane.一种贯穿人类红细胞膜的主要蛋白质。
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Microfilaments in cellular and developmental processes.细胞与发育过程中的微丝。
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Permeability and structure of junctional membranes at an electrotonic synapse.电突触处连接膜的通透性与结构
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10
Molecular localization of frog retinal receptor photopigment by electron microscopy and low-angle X-ray diffraction.通过电子显微镜和低角度X射线衍射对青蛙视网膜受体光色素进行分子定位
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人红细胞血影膜插入颗粒的平移流动性。pH依赖性可逆聚集。

Translational mobility of the membrane intercalated particles of human erythrocyte ghosts. pH-dependent, reversible aggregation.

作者信息

Pinto da Silva P

出版信息

J Cell Biol. 1972 Jun;53(3):777-87. doi: 10.1083/jcb.53.3.777.

DOI:10.1083/jcb.53.3.777
PMID:4554989
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2108782/
Abstract

This paper demonstrates the translational movement along the plane of the human erythrocyte ghost of the membrane particles exposed by freeze-fracture. The membrane particles can be aggregated by incubation of the ghosts in media with a pH in the vicinity of 5 5 or 3 5. The particles are disaggregated in neutral and alkaline media (pH 9 5) and also at pH 4.5 Aggregation of the particles at pH 5.5 is reversible, prevented by prefixation in glutaraldehyde and by media of high ionic strength. Particle aggregation occurs within 2-4 min. These results are consistent with the concept that the erythrocyte ghost membrane is a planar fluid domain formed by a bilayer membrane continuum which is interrupted by localized, yet mobile, proteic intercalations.

摘要

本文展示了通过冷冻断裂暴露的膜颗粒在人红细胞血影平面上的平移运动。通过将血影在pH值接近5.5或3.5的介质中孵育,膜颗粒可以聚集。在中性和碱性介质(pH 9.5)以及pH 4.5时,颗粒会解聚。在pH 5.5时颗粒的聚集是可逆的,戊二醛预固定和高离子强度介质可阻止这种聚集。颗粒聚集在2 - 4分钟内发生。这些结果与以下概念一致,即红细胞血影膜是由双层膜连续体形成的平面流体域,该连续体被局部但可移动的蛋白质插入物中断。