Oltmanns O, Bacher A
J Bacteriol. 1972 Jun;110(3):818-22. doi: 10.1128/jb.110.3.818-822.1972.
Haploid strains of Saccharomyces cerevisiae with mutations in two different rib genes were constructed. These strains were studied by tetrad analysis and by quantitative determination of accumulation products. The genes rib(1), rib(7), and rib(2) are not linked to each other. rib(1)-rib(7) strains and rib(1)-rib(2) strains exhibit the phenotypic properties of rib(1) strains. rib(7)-rib(2) strains show the phenotypic properties of rib(7) strains. The results support the conclusion that the genes rib(1) and rib(7) code for the first and second enzyme of the riboflavine pathway, respectively.
构建了在两个不同核糖体基因中发生突变的酿酒酵母单倍体菌株。通过四分体分析和对积累产物的定量测定对这些菌株进行了研究。核糖体基因rib(1)、rib(7)和rib(2)彼此不连锁。rib(1)-rib(7)菌株和rib(1)-rib(2)菌株表现出rib(1)菌株的表型特性。rib(7)-rib(2)菌株表现出rib(7)菌株的表型特性。这些结果支持了核糖体基因rib(1)和rib(7)分别编码核黄素途径的第一种和第二种酶的结论。