Muijsers A O, Van de Stadt R J, Henrichs A M, Van der Korst J K
Clin Chim Acta. 1979 Jun 1;94(2):173-80. doi: 10.1016/0009-8981(79)90010-x.
A chromatographic auto-analyser method is described for the determination of total D-penicillamine in biological fluids. After oxidation with performic acid, D-penicillaminic acid is separated from other ninhydrin-positive acidic amino acids by anion-exchange chromatography. Total cysteine/cystine is simultaneously determined as cysteic acid, which makes the method equally suitable for patients with cystinuria. The detection limit in serum and urine is 2 microM D-penicillamine, which is more than sufficient for clinical application. The metabolite S-methyl-D-penicillamine is oxidized by performic acid to a sulphone, which can be determined after separation by cation-exchange chromatography.
本文描述了一种用于测定生物体液中总 D-青霉胺的色谱自动分析仪方法。用过甲酸氧化后，通过阴离子交换色谱法将 D-青霉胺酸与其他茚三酮阳性酸性氨基酸分离。总半胱氨酸/胱氨酸同时以半胱氨酸磺酸形式测定，这使得该方法同样适用于胱氨酸尿症患者。血清和尿液中的检测限为 2 μM D-青霉胺，这对于临床应用来说绰绰有余。代谢物 S-甲基-D-青霉胺被过甲酸氧化成砜，可在通过阳离子交换色谱分离后进行测定。
